Abstract
Aim: The first aim was to determine whether hypermethylation of certain tumor related genes, including 30ST2, CHFR, RUNX3, and p16, could be detected in Japanese colorectal cancer (CRC) patients. The second was to utilize the most frequently hypermethylated genes as biomarkers for early detection of CRC. Materials and Methods: We investigated the aberrant promoter methylation profile of 4 genes in 129 colorectal tumors and corresponding normal mucosa. For the second step, bowel lavage and blood of the main tumor drainage vein from 21 CRC patients were analyzed. Results: The 30ST2 gene was the most frequently methylated (57%) and was methylated frequently (9/21, 43%) in bowel lavage but rarely (1/21; 4.7%) in the venous drainage. The concordance between 30ST2 methylation in bowel lavage and corresponding tumor samples was 71.4%. Conclusion: These results may show the possibility of early detection for CRC by examining 30ST2 gene methylation status in DNA extracted from stool.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4811-4818 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 30 |
| Issue number | 12 |
| State | Published - Dec 2010 |
Keywords
- 30ST2
- Blood of main drainage vein
- Bowel lavage
- Early detection
ASJC Scopus subject areas
- Oncology
- Cancer Research