ABF1-binding sites promote efficient global genome nucleotide excision repair

Shirong Yu, Julia B. Smirnova, Errol C. Friedberg, Bruce Stillman, Masahiro Akiyama, Tom Owen-Hughes, Raymond Waters, Simon H. Reed

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Global genome nucleotide excision repair (GG-NER) removes DNA damage from nontranscribing DNA. In Saccharomyces cerevisiae, the RAD7 and RAD16 genes are specifically required for GG-NER. We have reported that autonomously replicating sequence-binding factor 1 (ABF1) protein forms a stable complex with Rad7 and Rad16 proteins. ABF1 functions in transcription, replication, gene silencing, and NER in yeast. Here we show that binding of ABF1 to its DNA recognition sequence found at multiple genomic locations promotes efficient GG-NER in yeast. Mutation of the I silencer ABF1-binding site at the HMLα locus caused loss of ABF1 binding, which resulted in a domain of reduced GG-NER efficiency on one side of the ABF1-binding site. During GG-NER, nucleosome positioning at this site was not altered, and this correlated with an inability of the GG-NER complex to reposition nucleosomes in vitro.We discuss how the GG-NER complex might facilitate GG-NER while preventing unregulated gene transcription during this process.

Original languageEnglish (US)
Pages (from-to)966-973
Number of pages8
JournalJournal of Biological Chemistry
Volume284
Issue number2
DOIs
StatePublished - Jan 9 2009

Fingerprint

DNA Repair
Repair
Nucleotides
Genes
Binding Sites
Genome
Nucleosomes
Yeast
Transcription
Yeasts
DNA
Gene Silencing
DNA Damage
Saccharomyces cerevisiae
Proteins
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Yu, S., Smirnova, J. B., Friedberg, E. C., Stillman, B., Akiyama, M., Owen-Hughes, T., ... Reed, S. H. (2009). ABF1-binding sites promote efficient global genome nucleotide excision repair. Journal of Biological Chemistry, 284(2), 966-973. https://doi.org/10.1074/jbc.M806830200

ABF1-binding sites promote efficient global genome nucleotide excision repair. / Yu, Shirong; Smirnova, Julia B.; Friedberg, Errol C.; Stillman, Bruce; Akiyama, Masahiro; Owen-Hughes, Tom; Waters, Raymond; Reed, Simon H.

In: Journal of Biological Chemistry, Vol. 284, No. 2, 09.01.2009, p. 966-973.

Research output: Contribution to journalArticle

Yu, S, Smirnova, JB, Friedberg, EC, Stillman, B, Akiyama, M, Owen-Hughes, T, Waters, R & Reed, SH 2009, 'ABF1-binding sites promote efficient global genome nucleotide excision repair', Journal of Biological Chemistry, vol. 284, no. 2, pp. 966-973. https://doi.org/10.1074/jbc.M806830200
Yu, Shirong ; Smirnova, Julia B. ; Friedberg, Errol C. ; Stillman, Bruce ; Akiyama, Masahiro ; Owen-Hughes, Tom ; Waters, Raymond ; Reed, Simon H. / ABF1-binding sites promote efficient global genome nucleotide excision repair. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 2. pp. 966-973.
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