Ablation of Kv3.1 and Kv3.3 potassium channels disrupts thalamocortical oscillations in vitro and in vivo

Felipe Espinosa, Miguel A. Torres-Vega, Gerald A. Marks, Rolf H. Joho

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The genes Kcnc1 and Kcnc3 encode the subunits for the fast-activating/fast- deactivating, voltage-gated potassium channels Kv3.1 and Kv3.3, which are expressed in several brain regions known to be involved in the regulation of the sleep-wake cycle. When these genes are genetically eliminated, Kv3.1/Kv3.3-deficient mice display severe sleep loss as a result of unstable slow-wave sleep. Within the thalamocortical circuitry, Kv3.1 and Kv3.3 subunits are highly expressed in the thalamic reticular nucleus (TRN), which is thought to act as a pacemaker at sleep onset and to be involved in slow oscillatory activity (spindle waves) during slow-wave sleep. We showed that in cortical electroencephalographic recordings of freely moving Kv3.1/Kv3.3-deficient mice, spectral power is reduced up to70%at frequencies <15 Hz. In addition, the number of sleep spindles in vivo as well as rhythmic rebound firing of TRN neurons in vitro is diminished in mutant mice. Kv3.1/Kv3.3-deficient TRN neurons studied in vitro show ∼60% increase in action potential duration and a reduction in high-frequency firing after depolarizing current injections and during rebound burst firing. The results support the hypothesis that altered electrophysiological properties of TRN neurons contribute to the reduced EEG power at slow frequencies in the thalamocortical network of Kv3-deficient mice.

Original languageEnglish (US)
Pages (from-to)5570-5581
Number of pages12
JournalJournal of Neuroscience
Volume28
Issue number21
DOIs
StatePublished - May 21 2008

Fingerprint

Shaw Potassium Channels
Thalamic Nuclei
Sleep
Neurons
Voltage-Gated Potassium Channels
Genes
Action Potentials
In Vitro Techniques
Electroencephalography
Injections

Keywords

  • Cortical activation
  • Fast-spiking interneurons
  • Parvalbumin
  • Rhythmic rebound bursts
  • Spindle waves
  • Thalamic reticular nucleus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Ablation of Kv3.1 and Kv3.3 potassium channels disrupts thalamocortical oscillations in vitro and in vivo. / Espinosa, Felipe; Torres-Vega, Miguel A.; Marks, Gerald A.; Joho, Rolf H.

In: Journal of Neuroscience, Vol. 28, No. 21, 21.05.2008, p. 5570-5581.

Research output: Contribution to journalArticle

Espinosa, Felipe ; Torres-Vega, Miguel A. ; Marks, Gerald A. ; Joho, Rolf H. / Ablation of Kv3.1 and Kv3.3 potassium channels disrupts thalamocortical oscillations in vitro and in vivo. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 21. pp. 5570-5581.
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