Abnormal post-translational and extracellular processing of brevican in plaque-bearing mice over-expressing APPsw

Joanne M. Ajmo, Lauren A. Bailey, Matthew D. Howell, Lisa K. Cortez, Keith R. Pennypacker, Hina N. Mehta, Dave Morgan, Marcia N. Gordon, Paul E. Gottschall

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Aggregation of amyloid-β (Aβ) in the forebrain of Alzheimer's disease (AD) subjects may disturb the molecular organization of the extracellular microenvironment that modulates neural and synaptic plasticity. Proteoglycans are major components of this extracellular environment. To test the hypothesis that Aβ, or another amyloid precursor protein (APP) dependent mechanism modifies the accumulation and/or turnover of extracellular proteoglycans, we examined whether the expression and processing of brevican, an abundant extracellular, chondroitin sulfate (CS)-bearing proteoglycan, were altered in brains of Aβ-depositing transgenic mice (APPsw - APP gene bearing the Swedish mutation) as a model of AD. The molecular size of CS chains attached to brevican was smaller in hippocampal tissue from APPsw mice bearing Aβ deposits compared to non-transgenic mice, likely because of changes in the CS chains. Also, the abundance of the major proteolytic fragment of brevican was markedly diminished in extracts from several telencephalic regions of APPsw mice compared to non-transgenic mice, yet these immunoreactive fragments appeared to accumulate adjacent to the plaque edge. These results suggest that Aβ or APP exert inhibitory effects on proteolytic cleavage mechanisms responsible for synthesis and turnover of proteoglycans. As proteoglycans stabilize synaptic structure and inhibit molecular plasticity, defective brevican processing observed in Aβ-bearing mice and potentially end-stage human AD, may contribute to deficient neural plasticity.

Original languageEnglish (US)
Pages (from-to)784-795
Number of pages12
JournalJournal of Neurochemistry
Volume113
Issue number3
DOIs
StatePublished - May 1 2010

Keywords

  • ADAMTS
  • Alzheimer's disease
  • Amyloid β protein
  • Chondroitin sulfate
  • MMP
  • Proteoglycan

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Ajmo, J. M., Bailey, L. A., Howell, M. D., Cortez, L. K., Pennypacker, K. R., Mehta, H. N., Morgan, D., Gordon, M. N., & Gottschall, P. E. (2010). Abnormal post-translational and extracellular processing of brevican in plaque-bearing mice over-expressing APPsw. Journal of Neurochemistry, 113(3), 784-795. https://doi.org/10.1111/j.1471-4159.2010.06647.x