Absence of H 2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

An established cell line of murine teratoma cells (F9), which lacks serologically detectable H2 antigens, has a surface antigen structurally similar to H2 that is determined by a wild type T/t locus gene. These cells are not killed and do not react with cytotoxic T cells sensitized to H 2 antigens in a cell mediated lympholysis assay. Modification of spleen cells from the strain of origin (129) of this teratoma line with trinitrobenzene sulfonic acid allows the generation of syngeneic killer cells that display a cytotoxic effect against trinitrophenyl modified splenic targets, but not against trinitrophenyl modified F9 targets. Thus, the F9 antigen is structurally similar to H 2b but does not act as a target antigen in the cell mediated lympholysis assay for anti H 2b cytotoxic cells, nor does it crossreact with H 2b antigens at the T cell level.

Original languageEnglish (US)
Pages (from-to)3661-3665
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number9
StatePublished - 1975

Fingerprint

H-2 Antigens
Teratoma
T-Lymphocytes
Antigens
Trinitrobenzenes
Sulfonic Acids
Surface Antigens
Spleen
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{a2d1b81150b545d887dfc149a68d14e9,
title = "Absence of H 2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen",
abstract = "An established cell line of murine teratoma cells (F9), which lacks serologically detectable H2 antigens, has a surface antigen structurally similar to H2 that is determined by a wild type T/t locus gene. These cells are not killed and do not react with cytotoxic T cells sensitized to H 2 antigens in a cell mediated lympholysis assay. Modification of spleen cells from the strain of origin (129) of this teratoma line with trinitrobenzene sulfonic acid allows the generation of syngeneic killer cells that display a cytotoxic effect against trinitrophenyl modified splenic targets, but not against trinitrophenyl modified F9 targets. Thus, the F9 antigen is structurally similar to H 2b but does not act as a target antigen in the cell mediated lympholysis assay for anti H 2b cytotoxic cells, nor does it crossreact with H 2b antigens at the T cell level.",
author = "J. Forman and Vitetta, {E. S.}",
year = "1975",
language = "English (US)",
volume = "72",
pages = "3661--3665",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "9",

}

TY - JOUR

T1 - Absence of H 2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen

AU - Forman, J.

AU - Vitetta, E. S.

PY - 1975

Y1 - 1975

N2 - An established cell line of murine teratoma cells (F9), which lacks serologically detectable H2 antigens, has a surface antigen structurally similar to H2 that is determined by a wild type T/t locus gene. These cells are not killed and do not react with cytotoxic T cells sensitized to H 2 antigens in a cell mediated lympholysis assay. Modification of spleen cells from the strain of origin (129) of this teratoma line with trinitrobenzene sulfonic acid allows the generation of syngeneic killer cells that display a cytotoxic effect against trinitrophenyl modified splenic targets, but not against trinitrophenyl modified F9 targets. Thus, the F9 antigen is structurally similar to H 2b but does not act as a target antigen in the cell mediated lympholysis assay for anti H 2b cytotoxic cells, nor does it crossreact with H 2b antigens at the T cell level.

AB - An established cell line of murine teratoma cells (F9), which lacks serologically detectable H2 antigens, has a surface antigen structurally similar to H2 that is determined by a wild type T/t locus gene. These cells are not killed and do not react with cytotoxic T cells sensitized to H 2 antigens in a cell mediated lympholysis assay. Modification of spleen cells from the strain of origin (129) of this teratoma line with trinitrobenzene sulfonic acid allows the generation of syngeneic killer cells that display a cytotoxic effect against trinitrophenyl modified splenic targets, but not against trinitrophenyl modified F9 targets. Thus, the F9 antigen is structurally similar to H 2b but does not act as a target antigen in the cell mediated lympholysis assay for anti H 2b cytotoxic cells, nor does it crossreact with H 2b antigens at the T cell level.

UR - http://www.scopus.com/inward/record.url?scp=0016764369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016764369&partnerID=8YFLogxK

M3 - Article

C2 - 52873

AN - SCOPUS:0016764369

VL - 72

SP - 3661

EP - 3665

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 9

ER -