Absence of sensory neurons before target innervation in brain-derived neurotrophic factor-, neurotrophin 3-, and TrkC-deficient embryonic mice

Daniel J. Liebl, Lino Tessarollo, Mary Ellen Palko, Luis F. Parada

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Gene-targeting experiments of Trk receptors and neurotrophins has confirmed the expectation that embryonic sensory and sympathetic neurons require neurotrophin function for survival. They have further revealed correlation between a specific neurotrophin requirement and eventual sensory modality. We have analyzed embryonic and neonatal mice with mutations in the BDNF, neurotrophin 3 (NT-3), and TrkC genes. Our data confirm an unexpectedly high proportion of sensory neuron losses in NT-3 (>70%), BDNF (>20%), and TrkC (>30%) mutants, which encompass populations thought to be NGF-dependent. Direct comparison of TrkC and NT-3 mutants indicates that only a subset of the NT-3-dependent neurons also requires TrkC. The observed losses in our TrkC mutant, which is null for all proteins encoded by the gene, are more severe than those previously reported for the kinase-negative TrkC mutation, implicating additional and important functions for the truncated receptors. Our data further indicate that mature NGF-requiring neurons undergo precocious and transitory requirements for NT-3 and/or BDNF. We suggest that neurotrophins may function in creating early heterogeneity that would enable ganglia to compensate for diverse modality requirements before the period of naturally occurring death.

Original languageEnglish (US)
Pages (from-to)9113-9121
Number of pages9
JournalJournal of Neuroscience
Volume17
Issue number23
StatePublished - 1997

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Neurotrophin 3
Brain-Derived Neurotrophic Factor
Sensory Receptor Cells
Nerve Growth Factors
Nerve Growth Factor
Nerve Growth Factor Receptors
Neurons
Mutation
Gene Targeting
Ganglia
Phosphotransferases
Population
Genes
Proteins

Keywords

  • BDNF
  • Gene targeting
  • Neurotrophins
  • NT-3
  • Sensory neurons
  • Trk

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Absence of sensory neurons before target innervation in brain-derived neurotrophic factor-, neurotrophin 3-, and TrkC-deficient embryonic mice. / Liebl, Daniel J.; Tessarollo, Lino; Palko, Mary Ellen; Parada, Luis F.

In: Journal of Neuroscience, Vol. 17, No. 23, 1997, p. 9113-9121.

Research output: Contribution to journalArticle

Liebl, Daniel J. ; Tessarollo, Lino ; Palko, Mary Ellen ; Parada, Luis F. / Absence of sensory neurons before target innervation in brain-derived neurotrophic factor-, neurotrophin 3-, and TrkC-deficient embryonic mice. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 23. pp. 9113-9121.
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