Incubation of arachidonic acid with a reconstituted enzymatic system containing a purified preparation of the major, phenobarbital-inducible form of rat liver microsomal cytochrome P-450, NADPH, cytochrome b5, and NADPH-cytochrome P-450 reductase affords as the principal products four regioisomeric cis-epoxides: 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acids (EETs). Their absolute configurations were established by conversion to the corresponding hydroxyeicosatetraenoic acid (HETE) methyl esters, derivatization with dehydroabiethylisocyanate, and chromatographic analysis. Except for 5,6-EET, the cytochrome P-450 catalyzed epoxidation is highly enantioselective.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of the American Chemical Society|
|Publication status||Published - 1984|
ASJC Scopus subject areas