Absolute quantification of microrna from human and mouse tissue RNA using highly selective isotachophoretic focusing

Alexandre Persat, Raghu R. Chivukula, Joshua T. Mendell, Juan G. Santiago

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel technique for the quantification of small, non-coding RNA using selective isotachophoretic preconcentration and focusing. Our technique allows for absolute quantification of RNA shorter than 60 nt, in the size range of microRNA (miRNA). Our quantitation is extremely selective, requires only a single dispensing step on a standard chip, and quantifies oligomers <60 nt across five orders of magnitude. We demonstrate the efficacy and accuracy of our assay by quantifying miRNA from total RNA from diverse human and mouse tissues and cultured cells. Selective isotachophoresis (ITP) of miRNA is a unique and powerful method to measure miRNA expression data.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages659-661
Number of pages3
Volume1
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Fingerprint

RNA
Tissue
Oligomers
Assays
Cells

Keywords

  • Isotachophoresis
  • MicroRNA
  • Nucleic acids
  • Small RNA

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Persat, A., Chivukula, R. R., Mendell, J. T., & Santiago, J. G. (2010). Absolute quantification of microrna from human and mouse tissue RNA using highly selective isotachophoretic focusing. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 1, pp. 659-661)

Absolute quantification of microrna from human and mouse tissue RNA using highly selective isotachophoretic focusing. / Persat, Alexandre; Chivukula, Raghu R.; Mendell, Joshua T.; Santiago, Juan G.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 1 2010. p. 659-661.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Persat, A, Chivukula, RR, Mendell, JT & Santiago, JG 2010, Absolute quantification of microrna from human and mouse tissue RNA using highly selective isotachophoretic focusing. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 1, pp. 659-661, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/3/10.
Persat A, Chivukula RR, Mendell JT, Santiago JG. Absolute quantification of microrna from human and mouse tissue RNA using highly selective isotachophoretic focusing. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 1. 2010. p. 659-661
Persat, Alexandre ; Chivukula, Raghu R. ; Mendell, Joshua T. ; Santiago, Juan G. / Absolute quantification of microrna from human and mouse tissue RNA using highly selective isotachophoretic focusing. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 1 2010. pp. 659-661
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