TY - GEN
T1 - Acellular tissue engineering scaffolds for vocal fold regeneration
AU - Chan, Roger W.
PY - 2010
Y1 - 2010
N2 - A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold lamina propria, in order to develop an implantable tissue substitute that can promote a natural ECM remodeling response. A novel saline-based decellularization protocol developed in our laboratory was used to fabricate a threedimensional (3-D), biodegradable, acellular scaffold from native HUV tissue. Histological examination and scanning electron microscopy indicated that native cells in the HUV were removed with a fine 3-D structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were cultivated on the abluminal surface of the acellular scaffold in vitro. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These findings supported the biocompatibility of the HUV scaffold, and its promise for vocal fold reconstruction and regeneration.
AB - A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold lamina propria, in order to develop an implantable tissue substitute that can promote a natural ECM remodeling response. A novel saline-based decellularization protocol developed in our laboratory was used to fabricate a threedimensional (3-D), biodegradable, acellular scaffold from native HUV tissue. Histological examination and scanning electron microscopy indicated that native cells in the HUV were removed with a fine 3-D structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were cultivated on the abluminal surface of the acellular scaffold in vitro. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These findings supported the biocompatibility of the HUV scaffold, and its promise for vocal fold reconstruction and regeneration.
KW - Extracellular matrix scaffolds
KW - Larynx
KW - Vocal fold
UR - http://www.scopus.com/inward/record.url?scp=77956145590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956145590&partnerID=8YFLogxK
U2 - 10.1109/ICBBE.2010.5517382
DO - 10.1109/ICBBE.2010.5517382
M3 - Conference contribution
AN - SCOPUS:77956145590
SN - 9781424447138
T3 - 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
BT - 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
T2 - 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
Y2 - 18 June 2010 through 20 June 2010
ER -