Acellular tissue engineering scaffolds for vocal fold regeneration

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold lamina propria, in order to develop an implantable tissue substitute that can promote a natural ECM remodeling response. A novel saline-based decellularization protocol developed in our laboratory was used to fabricate a threedimensional (3-D), biodegradable, acellular scaffold from native HUV tissue. Histological examination and scanning electron microscopy indicated that native cells in the HUV were removed with a fine 3-D structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were cultivated on the abluminal surface of the acellular scaffold in vitro. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These findings supported the biocompatibility of the HUV scaffold, and its promise for vocal fold reconstruction and regeneration.

Original languageEnglish (US)
Title of host publication2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
DOIs
StatePublished - 2010
Event4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010 - Chengdu, China
Duration: Jun 18 2010Jun 20 2010

Other

Other4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
CountryChina
CityChengdu
Period6/18/106/20/10

Fingerprint

Tissue Scaffolds
Vocal Cords
Tissue Engineering
Scaffolds (biology)
Umbilical Veins
Tissue engineering
Regeneration
Scaffolds
Fibroblasts
Extracellular Matrix
Tissue
Bioelectric potentials
Biocompatibility
Infiltration
Proteoglycans
Electron Scanning Microscopy
Mucous Membrane
Proteins
Swine
Scanning electron microscopy

Keywords

  • Extracellular matrix scaffolds
  • Larynx
  • Vocal fold

ASJC Scopus subject areas

  • Biomedical Engineering
  • Health Informatics

Cite this

Chan, R. W. (2010). Acellular tissue engineering scaffolds for vocal fold regeneration. In 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010 [5517382] https://doi.org/10.1109/ICBBE.2010.5517382

Acellular tissue engineering scaffolds for vocal fold regeneration. / Chan, Roger W.

2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010. 2010. 5517382.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chan, RW 2010, Acellular tissue engineering scaffolds for vocal fold regeneration. in 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010., 5517382, 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010, Chengdu, China, 6/18/10. https://doi.org/10.1109/ICBBE.2010.5517382
Chan RW. Acellular tissue engineering scaffolds for vocal fold regeneration. In 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010. 2010. 5517382 https://doi.org/10.1109/ICBBE.2010.5517382
Chan, Roger W. / Acellular tissue engineering scaffolds for vocal fold regeneration. 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010. 2010.
@inproceedings{ef55984975df437d88eb4643fb5512b1,
title = "Acellular tissue engineering scaffolds for vocal fold regeneration",
abstract = "A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold lamina propria, in order to develop an implantable tissue substitute that can promote a natural ECM remodeling response. A novel saline-based decellularization protocol developed in our laboratory was used to fabricate a threedimensional (3-D), biodegradable, acellular scaffold from native HUV tissue. Histological examination and scanning electron microscopy indicated that native cells in the HUV were removed with a fine 3-D structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were cultivated on the abluminal surface of the acellular scaffold in vitro. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These findings supported the biocompatibility of the HUV scaffold, and its promise for vocal fold reconstruction and regeneration.",
keywords = "Extracellular matrix scaffolds, Larynx, Vocal fold",
author = "Chan, {Roger W.}",
year = "2010",
doi = "10.1109/ICBBE.2010.5517382",
language = "English (US)",
isbn = "9781424447138",
booktitle = "2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010",

}

TY - GEN

T1 - Acellular tissue engineering scaffolds for vocal fold regeneration

AU - Chan, Roger W.

PY - 2010

Y1 - 2010

N2 - A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold lamina propria, in order to develop an implantable tissue substitute that can promote a natural ECM remodeling response. A novel saline-based decellularization protocol developed in our laboratory was used to fabricate a threedimensional (3-D), biodegradable, acellular scaffold from native HUV tissue. Histological examination and scanning electron microscopy indicated that native cells in the HUV were removed with a fine 3-D structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were cultivated on the abluminal surface of the acellular scaffold in vitro. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These findings supported the biocompatibility of the HUV scaffold, and its promise for vocal fold reconstruction and regeneration.

AB - A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold lamina propria, in order to develop an implantable tissue substitute that can promote a natural ECM remodeling response. A novel saline-based decellularization protocol developed in our laboratory was used to fabricate a threedimensional (3-D), biodegradable, acellular scaffold from native HUV tissue. Histological examination and scanning electron microscopy indicated that native cells in the HUV were removed with a fine 3-D structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were cultivated on the abluminal surface of the acellular scaffold in vitro. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These findings supported the biocompatibility of the HUV scaffold, and its promise for vocal fold reconstruction and regeneration.

KW - Extracellular matrix scaffolds

KW - Larynx

KW - Vocal fold

UR - http://www.scopus.com/inward/record.url?scp=77956145590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956145590&partnerID=8YFLogxK

U2 - 10.1109/ICBBE.2010.5517382

DO - 10.1109/ICBBE.2010.5517382

M3 - Conference contribution

AN - SCOPUS:77956145590

SN - 9781424447138

BT - 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010

ER -