@article{2520b121ed1c425ea1ef1032da272970,
title = "Acetylation of HMG I(Y) by CBP turns off IFNβ expression by disrupting the enhanceosome",
abstract = "The transcriptional coactivators CBP and P/CAF are required for activation of transcription from the IFNβ enhanceosome. We show that CBP and P/CAF acetylate HMG I(Y), the essential architectural component required for enhanceosome assembly, at distinct lysine residues, causing distinct effects on transcription. Thus, in the context of the enhanceosome, acetylation of HMG I by CBP, but not by P/CAF, leads to enhanceosome destabilization and disassembly. We demonstrate that acetylation of HMG I(Y) by CBP is essential for turning off IFNβ gene expression. Finally, we show that the acetyltransferase activities of CBP and P/CAF modulate both the strength of the transcriptional response and the kinetics of virus-dependent activation of the IFNβ gene.",
author = "Nikhil Munshi and Menie Merika and Junming Yie and Kate Senger and Guoying Chen and Dimitris Thanos",
note = "Funding Information: We thank M. G. Rosenfeld for the CBP and P/CAF HAT − expression vectors, Y. Nakatani for the P/CAF expression vector, T. Kouzarides for GST-CBP expression vectors, J. Hiscott for IRF-3 plasmids, D. Levy for the IRF-7 plasmid, and A. Aggarwal and J. Carlson for P/CAF HAT domain protein. We also thank T. Maniatis, R. Mann, and T. Collins for critical reading and suggestions for the improvement of the manuscript. This work was supported by grants from the National Institute of Health (1RO1GM54605), the Pew Scholars Program in Biomedical Sciences, the March of Dimes, and the Irma T. Hirschl Foundation (to D. T.). N. M. was supported by the Columbia Medical Scientist Training Program (National Institutes of Health 5-T32-GM07367). K. S. was supported by a Training Grant for Careers in Molecular Ophthalmology (IT32 EY07105). ",
year = "1998",
month = oct,
doi = "10.1016/S1097-2765(00)80145-8",
language = "English (US)",
volume = "2",
pages = "457--467",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "4",
}