Acetylation of HMG I(Y) by CBP turns off IFNβ expression by disrupting the enhanceosome

Nikhil Munshi, Menie Merika, Junming Yie, Kate Senger, Guoying Chen, Dimitris Thanos

Research output: Contribution to journalArticle

296 Scopus citations

Abstract

The transcriptional coactivators CBP and P/CAF are required for activation of transcription from the IFNβ enhanceosome. We show that CBP and P/CAF acetylate HMG I(Y), the essential architectural component required for enhanceosome assembly, at distinct lysine residues, causing distinct effects on transcription. Thus, in the context of the enhanceosome, acetylation of HMG I by CBP, but not by P/CAF, leads to enhanceosome destabilization and disassembly. We demonstrate that acetylation of HMG I(Y) by CBP is essential for turning off IFNβ gene expression. Finally, we show that the acetyltransferase activities of CBP and P/CAF modulate both the strength of the transcriptional response and the kinetics of virus-dependent activation of the IFNβ gene.

Original languageEnglish (US)
Pages (from-to)457-467
Number of pages11
JournalMolecular cell
Volume2
Issue number4
DOIs
StatePublished - Oct 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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