TY - JOUR
T1 - ACh receptor-rich membrane domains organized in fibroblasts by recombinant 43-kildalton protein
AU - Phillips, William D.
AU - Kopta, Carrie
AU - Blount, Paul
AU - Gardner, Paul D.
AU - Steinbach, Joe Henry
AU - Merlie, John Paul
PY - 1991/2/1
Y1 - 1991/2/1
N2 - Neurotransmitter receptors are generally clustered in the postsynaptic membrane. The mechanism of clustering was analyzed with fibroblast cell lines that were stably transfected with the four subunits for fetal (α, β, γ, δ) or adult (α, β, ε, δ) type mouse muscle nicotinic acetylcholine receptors (AChRs). Immunofluorescent staining indicated that AChRs were dispersed on the surface of these cells. When transiently transfected with an expression construct encoding a 43-kilodalton protein that is normally concentrated under the postsynaptic membrane, AChRs expressed in these cells became aggregated in large cell-surface clusters, colocalized with the 43-kilodalton protein. This suggests that 43-kilodalton protein can induce AChR clustering and that cluster induction involves direct contact between AChR and 43-kilodalton protein.
AB - Neurotransmitter receptors are generally clustered in the postsynaptic membrane. The mechanism of clustering was analyzed with fibroblast cell lines that were stably transfected with the four subunits for fetal (α, β, γ, δ) or adult (α, β, ε, δ) type mouse muscle nicotinic acetylcholine receptors (AChRs). Immunofluorescent staining indicated that AChRs were dispersed on the surface of these cells. When transiently transfected with an expression construct encoding a 43-kilodalton protein that is normally concentrated under the postsynaptic membrane, AChRs expressed in these cells became aggregated in large cell-surface clusters, colocalized with the 43-kilodalton protein. This suggests that 43-kilodalton protein can induce AChR clustering and that cluster induction involves direct contact between AChR and 43-kilodalton protein.
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U2 - 10.1126/science.1703661
DO - 10.1126/science.1703661
M3 - Article
C2 - 1703661
AN - SCOPUS:0025829369
SN - 0036-8075
VL - 251
SP - 568
EP - 570
JO - Science
JF - Science
IS - 4993
ER -