Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence

S. M.Nashir Udden, M. Shamim Hasan Zahid, Kuntal Biswas, Qazi Shafi Ahmad, Alejandro Cravioto, G. Balakrish Nair, John J. Mekalanos, Shah M. Faruque

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The El Tor biotype of Vibrio cholerae O1, causing the current seventh pandemic of cholera, has replaced the classical biotype, which caused the sixth pandemic. The CTX prophages encoding cholera toxin in the two biotypes have distinct repressor (rstR) genes. Recently, new variants of El Tor strains that carry the classical type (CTXclass) prophage have emerged. These "hybrid" strains apparently originate through lateral gene transfer and recombination events. To explore possible donors of the CTXclass prophage and its mode of transfer, we tested environmental V. cholerae isolates for the presence of CTXclass prophage and mobility of the phage genome. Of the 272 environmental V. cholerae isolates tested, 6 were found to carry the CTXclass prophage; all of these belonged to the O141 serogroup. These O141 strains were unable to produce infectious CTX class phage or to transmit the prophage to recipient strains in the mouse model of infection; however, the CTXclass prophage was acquired by El Tor strains when cultured with the O141 strains in microcosms composed of filtered environmental water, a chitin substrate, and a V. cholerae O141-specific bacteriophage. The CTXclass prophage either coexisted with or replaced the resident CTXET prophage, resulting in El Tor strains with CTX genotypes similar to those of the naturally occurring hybrid strains. Our results support a model involving phages and natural chitin substrate in the emergence of new variants of pathogenic V. cholerae. Furthermore, the O141 strains apparently represent an alternative reservoir of the CTXclass phage genome, because the classical V. cholerae O1 strains are possibly extinct.

Original languageEnglish (US)
Pages (from-to)11951-11956
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number33
DOIs
StatePublished - Aug 19 2008
Externally publishedYes

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Prophages
Chitin
Vibrio cholerae
Bacteriophages
Mental Competency
Vibrio cholerae O1
Pandemics
Genome
Horizontal Gene Transfer
Cholera
Cholera Toxin
Genetic Recombination
Genotype

Keywords

  • Hybrid Vibrio cholerae strain
  • Toxigenic Vibrio cholerae

ASJC Scopus subject areas

  • General

Cite this

Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. / Udden, S. M.Nashir; Zahid, M. Shamim Hasan; Biswas, Kuntal; Ahmad, Qazi Shafi; Cravioto, Alejandro; Nair, G. Balakrish; Mekalanos, John J.; Faruque, Shah M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 33, 19.08.2008, p. 11951-11956.

Research output: Contribution to journalArticle

Udden, S. M.Nashir ; Zahid, M. Shamim Hasan ; Biswas, Kuntal ; Ahmad, Qazi Shafi ; Cravioto, Alejandro ; Nair, G. Balakrish ; Mekalanos, John J. ; Faruque, Shah M. / Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 33. pp. 11951-11956.
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AU - Ahmad, Qazi Shafi

AU - Cravioto, Alejandro

AU - Nair, G. Balakrish

AU - Mekalanos, John J.

AU - Faruque, Shah M.

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