Actin cytoskeleton reorganization by syk regulates fcγ receptor responsiveness by increasing its lateral mobility and clustering

Valentin Jaumouillé, Yoav Farkash, Khuloud Jaqaman, Raibatak Das, Clifford A. Lowell, Sergio Grinstein

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Clustering of immunoreceptors upon association with multivalent ligands triggers important responses including phagocytosis, secretion of cytokines, and production of immunoglobulins. We applied single-molecule detection and tracking methods to study the factors that control the mobility and clustering of phagocytic Fcγ receptors (FcγR). While the receptors exist as monomers in resting macrophages, two distinct populations were discernible based on their mobility: some diffuse by apparent free motion, while others are confined within submicron boundaries that reduce the frequency of spontaneous collisions. Src-family and Syk kinases determine the structure of the actin cytoskeleton, which is fenestrated, accounting for the heterogeneous diffusion of the FcγR. Stimulation of these kinases during phagocytosis induces reorganization of the cytoskeleton both locally and distally in a manner that alters receptor mobility and clustering, generating a feedback loop that facilitates engagement of FcγR at the tip of pseudopods, directing the progression of phagocytosis.

Original languageEnglish (US)
Pages (from-to)534-546
Number of pages13
JournalDevelopmental Cell
Volume29
Issue number5
DOIs
StatePublished - Jun 9 2014

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Fc Receptors
Actin Cytoskeleton
Phagocytosis
Cluster Analysis
Actins
Phosphotransferases
Pseudopodia
src-Family Kinases
Macrophages
Cytoskeleton
Immunoglobulins
Monomers
Cytokines
Ligands
Feedback
Molecules
Population

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Actin cytoskeleton reorganization by syk regulates fcγ receptor responsiveness by increasing its lateral mobility and clustering. / Jaumouillé, Valentin; Farkash, Yoav; Jaqaman, Khuloud; Das, Raibatak; Lowell, Clifford A.; Grinstein, Sergio.

In: Developmental Cell, Vol. 29, No. 5, 09.06.2014, p. 534-546.

Research output: Contribution to journalArticle

Jaumouillé, Valentin ; Farkash, Yoav ; Jaqaman, Khuloud ; Das, Raibatak ; Lowell, Clifford A. ; Grinstein, Sergio. / Actin cytoskeleton reorganization by syk regulates fcγ receptor responsiveness by increasing its lateral mobility and clustering. In: Developmental Cell. 2014 ; Vol. 29, No. 5. pp. 534-546.
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