Actin Homolog MreB Affects Chromosome Segregation by Regulating Topoisomerase IV in Escherichia coli

Ram Madabhushi, Kenneth J. Marians

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this temporal regulation is unknown. Here we report that the bacterial actin homolog MreB participates in the temporal oscillation of Topo IV activity. We show that mreB mutant strains are deficient in Topo IV activity. In addition, we demonstrate that, depending upon whether it is in a monomeric or polymerized state, MreB affects Topo IV activity differentially. In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation.

Original languageEnglish (US)
Pages (from-to)171-180
Number of pages10
JournalMolecular Cell
Volume33
Issue number2
DOIs
StatePublished - Jan 30 2009

Fingerprint

DNA Topoisomerase IV
Chromosome Segregation
Actins
Escherichia coli
Type II DNA Topoisomerase
Cytoskeleton
Cell Cycle
Chromosomes

Keywords

  • CELLCYCLE
  • DNA
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Actin Homolog MreB Affects Chromosome Segregation by Regulating Topoisomerase IV in Escherichia coli. / Madabhushi, Ram; Marians, Kenneth J.

In: Molecular Cell, Vol. 33, No. 2, 30.01.2009, p. 171-180.

Research output: Contribution to journalArticle

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