Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster

Naren Srinivasan, Oliver Gordon, Susan Ahrens, Anna Franz, Safia Deddouche, Probir Chakravarty, David Phillips, Ali A. Yunus, Michael K. Rosen, Rita S. Valente, Luis Teixeira, Barry Thompson, Marc S. Dionne, Will Wood, Caetano Reis e Sousa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Damage-associated molecular patterns (DAMPs) are molecules released by dead cells that trigger sterile inflammation and, in vertebrates, adaptive immunity. Actin is a DAMP detected in mammals by the receptor, DNGR-1, expressed by dendritic cells (DCs). DNGR-1 is phosphorylated by Src-family kinases and recruits the tyrosine kinase Syk to promote DC cross- presentation of dead cell-associated antigens. Here we report that actin is also a DAMP in invertebrates that lack DCs and adaptive immunity. Administration of actin to Drosophila melanogaster triggers a response characterised by selective induction of STAT target genes in the fat body through the cytokine Upd3 and its JAK/STAT-coupled receptor, Domeless. Notably, this response requires signalling via Shark, the Drosophila orthologue of Syk, and Src42A, a Drosophila Src-family kinase, and is dependent on Nox activity. Thus, extracellular actin detection via a Src- family kinase-dependent cascade is an ancient means of detecting cell injury that precedes the evolution of adaptive immunity.

Original languageEnglish (US)
Article numbere19662
JournaleLife
Volume5
Issue numberNOVEMBER2016
DOIs
StatePublished - Nov 22 2016

Fingerprint

Drosophila melanogaster
src-Family Kinases
Actins
Adaptive Immunity
Dendritic Cells
Tissue
Wounds and Injuries
Drosophila
Cross-Priming
Sharks
Mammals
Fat Body
Invertebrates
Protein-Tyrosine Kinases
Vertebrates
Genes
Fats
Cytokines
Inflammation
Antigens

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Srinivasan, N., Gordon, O., Ahrens, S., Franz, A., Deddouche, S., Chakravarty, P., ... Reis e Sousa, C. (2016). Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster. eLife, 5(NOVEMBER2016), [e19662]. https://doi.org/10.7554/eLife.19662

Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster. / Srinivasan, Naren; Gordon, Oliver; Ahrens, Susan; Franz, Anna; Deddouche, Safia; Chakravarty, Probir; Phillips, David; Yunus, Ali A.; Rosen, Michael K.; Valente, Rita S.; Teixeira, Luis; Thompson, Barry; Dionne, Marc S.; Wood, Will; Reis e Sousa, Caetano.

In: eLife, Vol. 5, No. NOVEMBER2016, e19662, 22.11.2016.

Research output: Contribution to journalArticle

Srinivasan, N, Gordon, O, Ahrens, S, Franz, A, Deddouche, S, Chakravarty, P, Phillips, D, Yunus, AA, Rosen, MK, Valente, RS, Teixeira, L, Thompson, B, Dionne, MS, Wood, W & Reis e Sousa, C 2016, 'Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster', eLife, vol. 5, no. NOVEMBER2016, e19662. https://doi.org/10.7554/eLife.19662
Srinivasan N, Gordon O, Ahrens S, Franz A, Deddouche S, Chakravarty P et al. Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster. eLife. 2016 Nov 22;5(NOVEMBER2016). e19662. https://doi.org/10.7554/eLife.19662
Srinivasan, Naren ; Gordon, Oliver ; Ahrens, Susan ; Franz, Anna ; Deddouche, Safia ; Chakravarty, Probir ; Phillips, David ; Yunus, Ali A. ; Rosen, Michael K. ; Valente, Rita S. ; Teixeira, Luis ; Thompson, Barry ; Dionne, Marc S. ; Wood, Will ; Reis e Sousa, Caetano. / Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster. In: eLife. 2016 ; Vol. 5, No. NOVEMBER2016.
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