Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility

Patricia T. Yam, Cyrus A. Wilson, Lin Ji, Benedict Hebert, Erin L. Barnhart, Natalie A. Dye, Paul W. Wiseman, Gaudenz Danuser, Julie A. Theriot

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

We have analyzed the spontaneous symmetry breaking and initiation of actin-based motility in keratocytes (fish epithelial cells). In stationary keratocytes, the actin network flow was inwards and radially symmetric. Immediately before motility initiation, the actin network flow increased at the prospective cell rear and reoriented in the perinuclear region, aligning with the prospective axis of movement. Changes in actin network flow at the cell front were detectable only after cell polarization. Inhibition of myosin II or Rho kinase disrupted actin network organization and flow in the perinuclear region and decreased the motility initiation frequency, whereas increasing myosin II activity with calyculin A increased the motility initiation frequency. Local stimulation of myosin activity in stationary cells by the local application of calyculin A induced directed motility initiation away from the site of stimulation. Together, these results indicate that large-scale actin-myosin network reorganization and contractility at the cell rear initiate spontaneous symmetry breaking and polarized motility of keratocytes.

Original languageEnglish (US)
Pages (from-to)1207-1221
Number of pages15
JournalJournal of Cell Biology
Volume178
Issue number7
DOIs
StatePublished - Sep 24 2007

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Myosins
Cell Movement
Actins
Myosin Type II
Myosin-Light-Chain Kinase
rho-Associated Kinases
Fishes
Epithelial Cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Yam, P. T., Wilson, C. A., Ji, L., Hebert, B., Barnhart, E. L., Dye, N. A., ... Theriot, J. A. (2007). Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. Journal of Cell Biology, 178(7), 1207-1221. https://doi.org/10.1083/jcb.200706012

Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. / Yam, Patricia T.; Wilson, Cyrus A.; Ji, Lin; Hebert, Benedict; Barnhart, Erin L.; Dye, Natalie A.; Wiseman, Paul W.; Danuser, Gaudenz; Theriot, Julie A.

In: Journal of Cell Biology, Vol. 178, No. 7, 24.09.2007, p. 1207-1221.

Research output: Contribution to journalArticle

Yam, PT, Wilson, CA, Ji, L, Hebert, B, Barnhart, EL, Dye, NA, Wiseman, PW, Danuser, G & Theriot, JA 2007, 'Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility', Journal of Cell Biology, vol. 178, no. 7, pp. 1207-1221. https://doi.org/10.1083/jcb.200706012
Yam, Patricia T. ; Wilson, Cyrus A. ; Ji, Lin ; Hebert, Benedict ; Barnhart, Erin L. ; Dye, Natalie A. ; Wiseman, Paul W. ; Danuser, Gaudenz ; Theriot, Julie A. / Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. In: Journal of Cell Biology. 2007 ; Vol. 178, No. 7. pp. 1207-1221.
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