Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion

Khurts Shilagardi, Shuo Li, Fengbao Luo, Faiz Marikar, Rui Duan, Peng Jin, Ji Hoon Kim, Katherine Murnen, Elizabeth Chen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Cell-cell fusion is critical for the conception, development, and physiology of multicellular organisms. Although cellular fusogenic proteins and the actin cytoskeleton are implicated in cell-cell fusion, it remains unclear whether and how they coordinate to promote plasma membrane fusion. We reconstituted a high-efficiency, inducible cell fusion culture system in the normally nonfusing Drosophila S2R+ cells. Both fusogenic proteins and actin cytoskeletal rearrangements were necessary for cell fusion, and in combination they were sufficient to impart fusion competence. Localized actin polymerization triggered by specific cell-cell or cell-matrix adhesion molecules propelled invasive cell membrane protrusions, which in turn promoted fusogenic protein engagement and plasma membrane fusion. This de novo cell fusion culture system reveals a general role for actin-propelled invasive membrane protrusions in driving fusogenic protein engagement during cell-cell fusion.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalScience
Volume340
Issue number6130
DOIs
StatePublished - Apr 19 2013

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Cell Fusion
Actins
Membranes
Proteins
Membrane Fusion
Cell Surface Extensions
Cell Culture Techniques
Cell Membrane
Cell-Matrix Junctions
Cytoskeletal Proteins
Cell Adhesion Molecules
Actin Cytoskeleton
Polymerization
Mental Competency
Drosophila

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion. / Shilagardi, Khurts; Li, Shuo; Luo, Fengbao; Marikar, Faiz; Duan, Rui; Jin, Peng; Kim, Ji Hoon; Murnen, Katherine; Chen, Elizabeth.

In: Science, Vol. 340, No. 6130, 19.04.2013, p. 359-363.

Research output: Contribution to journalArticle

Shilagardi, K, Li, S, Luo, F, Marikar, F, Duan, R, Jin, P, Kim, JH, Murnen, K & Chen, E 2013, 'Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion', Science, vol. 340, no. 6130, pp. 359-363. https://doi.org/10.1126/science.1234781
Shilagardi, Khurts ; Li, Shuo ; Luo, Fengbao ; Marikar, Faiz ; Duan, Rui ; Jin, Peng ; Kim, Ji Hoon ; Murnen, Katherine ; Chen, Elizabeth. / Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion. In: Science. 2013 ; Vol. 340, No. 6130. pp. 359-363.
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