Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion

Khurts Shilagardi, Shuo Li, Fengbao Luo, Faiz Marikar, Rui Duan, Peng Jin, Ji Hoon Kim, Katherine Murnen, Elizabeth Chen

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Abstract

Cell-cell fusion is critical for the conception, development, and physiology of multicellular organisms. Although cellular fusogenic proteins and the actin cytoskeleton are implicated in cell-cell fusion, it remains unclear whether and how they coordinate to promote plasma membrane fusion. We reconstituted a high-efficiency, inducible cell fusion culture system in the normally nonfusing Drosophila S2R+ cells. Both fusogenic proteins and actin cytoskeletal rearrangements were necessary for cell fusion, and in combination they were sufficient to impart fusion competence. Localized actin polymerization triggered by specific cell-cell or cell-matrix adhesion molecules propelled invasive cell membrane protrusions, which in turn promoted fusogenic protein engagement and plasma membrane fusion. This de novo cell fusion culture system reveals a general role for actin-propelled invasive membrane protrusions in driving fusogenic protein engagement during cell-cell fusion.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalScience
Volume340
Issue number6130
DOIs
StatePublished - Apr 19 2013

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ASJC Scopus subject areas

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Cite this

Shilagardi, K., Li, S., Luo, F., Marikar, F., Duan, R., Jin, P., Kim, J. H., Murnen, K., & Chen, E. (2013). Actin-propelled invasive membrane protrusions promote fusogenic protein engagement during cell-cell fusion. Science, 340(6130), 359-363. https://doi.org/10.1126/science.1234781