Action of brefeldin A blocked by activation of a pertussistoxin-sensitive G protein

Nicholas T. Ktistakis, Maurine E. Linder, Michael G. Roth

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

IN many mammalian cells brefeldin A interferes with mechanisms that keep the Golgi apparatus separate from the endoplasmic reticulum1-8. The earliest effect of brefeldin A is release of the coat protein β-COP from the Golgi9-11. This release is blocked by pretreatment with GTP-γS or AlF4- (ref. 12). The AlF4- ion activates heterotrimeric G proteins13 but not proteins of the ras superfamily14, suggesting that a heterotrimeric G protein might control membrane transfer from the endoplasmic reticulum to the Golgi. We report here that mastoparan, a peptide that activates heterotrimeric G proteins15,16, promotes binding of β-COP to Golgi membranes in vitro and antagonizes the effect of brefeldin A on β-COP in perforated cells and on isolated Golgi membranes. This inhibition is greatly diminished if cells are pretreated with pertussis toxin before perforation., Thus, a heterotrimeric G protein of the Gi/Go subfamily regulates association of coat components with Golgi membranes.

Original languageEnglish (US)
Pages (from-to)344-346
Number of pages3
JournalNature
Volume356
Issue number6367
StatePublished - Mar 26 1992

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Brefeldin A
GTP-Binding Proteins
Heterotrimeric GTP-Binding Proteins
Membranes
Gi-Go GTP-Binding Protein alpha Subunits
ras Proteins
Pertussis Toxin
Capsid Proteins
Golgi Apparatus
Guanosine Triphosphate
Endoplasmic Reticulum
Peptides
tetrafluoroaluminate

ASJC Scopus subject areas

  • General

Cite this

Action of brefeldin A blocked by activation of a pertussistoxin-sensitive G protein. / Ktistakis, Nicholas T.; Linder, Maurine E.; Roth, Michael G.

In: Nature, Vol. 356, No. 6367, 26.03.1992, p. 344-346.

Research output: Contribution to journalArticle

Ktistakis, NT, Linder, ME & Roth, MG 1992, 'Action of brefeldin A blocked by activation of a pertussistoxin-sensitive G protein', Nature, vol. 356, no. 6367, pp. 344-346.
Ktistakis, Nicholas T. ; Linder, Maurine E. ; Roth, Michael G. / Action of brefeldin A blocked by activation of a pertussistoxin-sensitive G protein. In: Nature. 1992 ; Vol. 356, No. 6367. pp. 344-346.
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AB - IN many mammalian cells brefeldin A interferes with mechanisms that keep the Golgi apparatus separate from the endoplasmic reticulum1-8. The earliest effect of brefeldin A is release of the coat protein β-COP from the Golgi9-11. This release is blocked by pretreatment with GTP-γS or AlF4- (ref. 12). The AlF4- ion activates heterotrimeric G proteins13 but not proteins of the ras superfamily14, suggesting that a heterotrimeric G protein might control membrane transfer from the endoplasmic reticulum to the Golgi. We report here that mastoparan, a peptide that activates heterotrimeric G proteins15,16, promotes binding of β-COP to Golgi membranes in vitro and antagonizes the effect of brefeldin A on β-COP in perforated cells and on isolated Golgi membranes. This inhibition is greatly diminished if cells are pretreated with pertussis toxin before perforation., Thus, a heterotrimeric G protein of the Gi/Go subfamily regulates association of coat components with Golgi membranes.

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