Action of lead on catecholamine secretory processes of fetal hypothalamic and adrenal cells

Susan M. Ramin, Wojciech Kedzierski, John C. Porter

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effects of lead nitrate [Pb(NO3)2] on tyrosine hydroxylase (TH) mass, TH mRNA, and catecholamine (CA) secretion by primary cultures of hypothalamic and adrenal cells of 18- to 22-day-old rat fetuses were investigated. Two-week-old hypothalamic and 4-week-old adrenal cell cultures were incubated for 24 h or 14 days with various concentrations (10-10 to 10-3 M) of Pb(NO3)2. In general, a 24-h exposure to low concentrations of Pb (10-10 to 10-7 M) suppressed the secretion of dihydroxyphenylalanine (DOPA) by hypothalamic dopaminergic cells and especially norepinephrine and epinephrine by adrenal cells; conversely, high concentrations (10-4 and 10-3 M) were stimulatory. This biphasic, concentration-dependent action of Pb on CA secretion occurred irrespective of the gestational age of the cell donor. Long-term (14 days) incubation of hypothalamic cells with Pb had an effect similar to that seen with a short-term incubation except that 10-3 M Pb inhibited DOPA secretion. For adrenal cells, a long-term incubation with 10-10 and 10-9 M Pb inhibited CA secretion, whereas 10-8 M was stimulatory. The TH mRNA content of the cells was not affected by Pb(NO3)2, regardless of its concentration. However, a 14-day incubation with low concentrations (10-10 and 10-9 M) of Pb resulted in an increase in TH mass in hypothalamic cells compared to controls, whereas high concentrations (10-4 and 10-3 M) markedly suppressed the level of TH. The effects of Pb on TH in adrenal cells were similar to those in hypothalamic cells. In conclusion, the actions of Pb(NO3)2 on CA secretion are biphasic: low concentrations are inhibitory, and high concentrations are stimulatory. Time of exposure does not appear to be a significant factor since a 24-h incubation with Pb was as effective as was a 14-day incubation. Thus, Pb(NO3)2 has a paradoxical effect on catecholaminergic cells, and in the absence of knowledge of the concentration of Pb, it is not possible to predict the toxic consequences of lead poisoning.

Original languageEnglish (US)
Pages (from-to)449-454
Number of pages6
JournalMolecular and Cellular Neuroscience
Volume4
Issue number5
StatePublished - 1993

Fingerprint

Secretory Pathway
Catecholamines
Tyrosine 3-Monooxygenase
Dihydroxyphenylalanine
Lead
Lead Poisoning
Messenger RNA
Poisons
Epinephrine
Gestational Age
Norepinephrine
Fetus
Cell Culture Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Action of lead on catecholamine secretory processes of fetal hypothalamic and adrenal cells. / Ramin, Susan M.; Kedzierski, Wojciech; Porter, John C.

In: Molecular and Cellular Neuroscience, Vol. 4, No. 5, 1993, p. 449-454.

Research output: Contribution to journalArticle

Ramin, Susan M. ; Kedzierski, Wojciech ; Porter, John C. / Action of lead on catecholamine secretory processes of fetal hypothalamic and adrenal cells. In: Molecular and Cellular Neuroscience. 1993 ; Vol. 4, No. 5. pp. 449-454.
@article{d06fcfb9c32841c487a56a5cec6c350c,
title = "Action of lead on catecholamine secretory processes of fetal hypothalamic and adrenal cells",
abstract = "The effects of lead nitrate [Pb(NO3)2] on tyrosine hydroxylase (TH) mass, TH mRNA, and catecholamine (CA) secretion by primary cultures of hypothalamic and adrenal cells of 18- to 22-day-old rat fetuses were investigated. Two-week-old hypothalamic and 4-week-old adrenal cell cultures were incubated for 24 h or 14 days with various concentrations (10-10 to 10-3 M) of Pb(NO3)2. In general, a 24-h exposure to low concentrations of Pb (10-10 to 10-7 M) suppressed the secretion of dihydroxyphenylalanine (DOPA) by hypothalamic dopaminergic cells and especially norepinephrine and epinephrine by adrenal cells; conversely, high concentrations (10-4 and 10-3 M) were stimulatory. This biphasic, concentration-dependent action of Pb on CA secretion occurred irrespective of the gestational age of the cell donor. Long-term (14 days) incubation of hypothalamic cells with Pb had an effect similar to that seen with a short-term incubation except that 10-3 M Pb inhibited DOPA secretion. For adrenal cells, a long-term incubation with 10-10 and 10-9 M Pb inhibited CA secretion, whereas 10-8 M was stimulatory. The TH mRNA content of the cells was not affected by Pb(NO3)2, regardless of its concentration. However, a 14-day incubation with low concentrations (10-10 and 10-9 M) of Pb resulted in an increase in TH mass in hypothalamic cells compared to controls, whereas high concentrations (10-4 and 10-3 M) markedly suppressed the level of TH. The effects of Pb on TH in adrenal cells were similar to those in hypothalamic cells. In conclusion, the actions of Pb(NO3)2 on CA secretion are biphasic: low concentrations are inhibitory, and high concentrations are stimulatory. Time of exposure does not appear to be a significant factor since a 24-h incubation with Pb was as effective as was a 14-day incubation. Thus, Pb(NO3)2 has a paradoxical effect on catecholaminergic cells, and in the absence of knowledge of the concentration of Pb, it is not possible to predict the toxic consequences of lead poisoning.",
author = "Ramin, {Susan M.} and Wojciech Kedzierski and Porter, {John C.}",
year = "1993",
language = "English (US)",
volume = "4",
pages = "449--454",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Action of lead on catecholamine secretory processes of fetal hypothalamic and adrenal cells

AU - Ramin, Susan M.

AU - Kedzierski, Wojciech

AU - Porter, John C.

PY - 1993

Y1 - 1993

N2 - The effects of lead nitrate [Pb(NO3)2] on tyrosine hydroxylase (TH) mass, TH mRNA, and catecholamine (CA) secretion by primary cultures of hypothalamic and adrenal cells of 18- to 22-day-old rat fetuses were investigated. Two-week-old hypothalamic and 4-week-old adrenal cell cultures were incubated for 24 h or 14 days with various concentrations (10-10 to 10-3 M) of Pb(NO3)2. In general, a 24-h exposure to low concentrations of Pb (10-10 to 10-7 M) suppressed the secretion of dihydroxyphenylalanine (DOPA) by hypothalamic dopaminergic cells and especially norepinephrine and epinephrine by adrenal cells; conversely, high concentrations (10-4 and 10-3 M) were stimulatory. This biphasic, concentration-dependent action of Pb on CA secretion occurred irrespective of the gestational age of the cell donor. Long-term (14 days) incubation of hypothalamic cells with Pb had an effect similar to that seen with a short-term incubation except that 10-3 M Pb inhibited DOPA secretion. For adrenal cells, a long-term incubation with 10-10 and 10-9 M Pb inhibited CA secretion, whereas 10-8 M was stimulatory. The TH mRNA content of the cells was not affected by Pb(NO3)2, regardless of its concentration. However, a 14-day incubation with low concentrations (10-10 and 10-9 M) of Pb resulted in an increase in TH mass in hypothalamic cells compared to controls, whereas high concentrations (10-4 and 10-3 M) markedly suppressed the level of TH. The effects of Pb on TH in adrenal cells were similar to those in hypothalamic cells. In conclusion, the actions of Pb(NO3)2 on CA secretion are biphasic: low concentrations are inhibitory, and high concentrations are stimulatory. Time of exposure does not appear to be a significant factor since a 24-h incubation with Pb was as effective as was a 14-day incubation. Thus, Pb(NO3)2 has a paradoxical effect on catecholaminergic cells, and in the absence of knowledge of the concentration of Pb, it is not possible to predict the toxic consequences of lead poisoning.

AB - The effects of lead nitrate [Pb(NO3)2] on tyrosine hydroxylase (TH) mass, TH mRNA, and catecholamine (CA) secretion by primary cultures of hypothalamic and adrenal cells of 18- to 22-day-old rat fetuses were investigated. Two-week-old hypothalamic and 4-week-old adrenal cell cultures were incubated for 24 h or 14 days with various concentrations (10-10 to 10-3 M) of Pb(NO3)2. In general, a 24-h exposure to low concentrations of Pb (10-10 to 10-7 M) suppressed the secretion of dihydroxyphenylalanine (DOPA) by hypothalamic dopaminergic cells and especially norepinephrine and epinephrine by adrenal cells; conversely, high concentrations (10-4 and 10-3 M) were stimulatory. This biphasic, concentration-dependent action of Pb on CA secretion occurred irrespective of the gestational age of the cell donor. Long-term (14 days) incubation of hypothalamic cells with Pb had an effect similar to that seen with a short-term incubation except that 10-3 M Pb inhibited DOPA secretion. For adrenal cells, a long-term incubation with 10-10 and 10-9 M Pb inhibited CA secretion, whereas 10-8 M was stimulatory. The TH mRNA content of the cells was not affected by Pb(NO3)2, regardless of its concentration. However, a 14-day incubation with low concentrations (10-10 and 10-9 M) of Pb resulted in an increase in TH mass in hypothalamic cells compared to controls, whereas high concentrations (10-4 and 10-3 M) markedly suppressed the level of TH. The effects of Pb on TH in adrenal cells were similar to those in hypothalamic cells. In conclusion, the actions of Pb(NO3)2 on CA secretion are biphasic: low concentrations are inhibitory, and high concentrations are stimulatory. Time of exposure does not appear to be a significant factor since a 24-h incubation with Pb was as effective as was a 14-day incubation. Thus, Pb(NO3)2 has a paradoxical effect on catecholaminergic cells, and in the absence of knowledge of the concentration of Pb, it is not possible to predict the toxic consequences of lead poisoning.

UR - http://www.scopus.com/inward/record.url?scp=0027363201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027363201&partnerID=8YFLogxK

M3 - Article

C2 - 19912952

AN - SCOPUS:0027363201

VL - 4

SP - 449

EP - 454

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 5

ER -