Activated eosinophils upregulate the metastasis suppressor molecule E-cadherin on prostate tumor cells.

P. M. Furbert-Harris, D. Parish-Gause, K. A. Hunter, T. R. Vaughn, C. Howland, J. Okomo-Awich, K. Forrest, I. Laniyan, A. Abdelnaby, O. A. Oredipe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cell adhesion molecules (CAMs) play an important role in cancer metastasis by facilitating attachment to vascular endothelia, invasion and spread into secondary tissue sites. We have shown that activated eosinophils (EosA) inhibited the growth of prostate cancer (Pca) cells in vitro. In the present study, we examined the ability of EosA 24 hr conditioned supernatants (EosAcs) to modulate the expression of ICAM-1, VCAM-1, ELAM-1, E-cadherin and N-cadherin expression on human Pca cell lines, Du-145 and PC-3 by flow cytometry. TNF-alpha, IL-10 and IL-12 were also evaluated. ICAM-1, expressed on PC-3 and DU 145 cells, was enhanced by TNF-alpha and IL-10. ELAM-1 was present on DU 145 cells but absent on PC-3. TNF-alpha and IL-10 enhanced ELAM-1 on DU 145, but EosA 24 hr supematants failed to do so. All three cytokines, namely IL-10, IL-12 and TNF-alpha-induced ELAM-1 on PC-3 tumor cells. Although VCAM-1 was absent on DU 145 and PC-3 cells, it was expressed on DU-145 cells after exposure to EosA: tumor cell co-cultures, and was expressed on PC-3 following exposure to IL-10 and IL-12. N-cadherin and E-cadherin were both expressed on DU-145. While N-cadherin was expressed on PC-3 cells, E-cadherin was not. N-cadherin was enhanced on DU-145 and PC-3 cells following exposure to EosA co-culture and upregulated on PC-3 by IL-10 and EosA 24 hr supernatants, but decreased by IL-12. E-cadherin was up-regulated on DU 145 cells following co-culture with EosA and was induced on PC-3 by IL-10 and IL-12, but not by EosA co-culture and 24 hr supematants. In conclusion, inflammatory and non-inflammatory cytokines modulate CAM expression on Pca cells; EosA and EosA 24 hr supernatants also exerted modulatory activity of CAM expression. Most significantly, the metastasis suppressor molecule, E-cadherin was enhanced on DU 145 cells by EosA and induced on PC-3 by IL-10 and IL-12 both of which are produced by EosA. This suggests potential use of these cytokines in immunotherapeutic strategies for prostate cancer and its metastasis.

Original languageEnglish (US)
Pages (from-to)1009-1016
Number of pages8
JournalCellular and molecular biology (Noisy-le-Grand, France)
Volume49
Issue number7
StatePublished - Nov 2003

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Cadherins
Eosinophils
Tumors
Prostate
Interleukin-10
Up-Regulation
Cells
Interleukin-12
Neoplasm Metastasis
Molecules
E-Selectin
Neoplasms
Coculture Techniques
Cell Adhesion Molecules
Tumor Necrosis Factor-alpha
Prostatic Neoplasms
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Cytokines
Flow cytometry

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Furbert-Harris, P. M., Parish-Gause, D., Hunter, K. A., Vaughn, T. R., Howland, C., Okomo-Awich, J., ... Oredipe, O. A. (2003). Activated eosinophils upregulate the metastasis suppressor molecule E-cadherin on prostate tumor cells. Cellular and molecular biology (Noisy-le-Grand, France), 49(7), 1009-1016.

Activated eosinophils upregulate the metastasis suppressor molecule E-cadherin on prostate tumor cells. / Furbert-Harris, P. M.; Parish-Gause, D.; Hunter, K. A.; Vaughn, T. R.; Howland, C.; Okomo-Awich, J.; Forrest, K.; Laniyan, I.; Abdelnaby, A.; Oredipe, O. A.

In: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 49, No. 7, 11.2003, p. 1009-1016.

Research output: Contribution to journalArticle

Furbert-Harris, PM, Parish-Gause, D, Hunter, KA, Vaughn, TR, Howland, C, Okomo-Awich, J, Forrest, K, Laniyan, I, Abdelnaby, A & Oredipe, OA 2003, 'Activated eosinophils upregulate the metastasis suppressor molecule E-cadherin on prostate tumor cells.', Cellular and molecular biology (Noisy-le-Grand, France), vol. 49, no. 7, pp. 1009-1016.
Furbert-Harris PM, Parish-Gause D, Hunter KA, Vaughn TR, Howland C, Okomo-Awich J et al. Activated eosinophils upregulate the metastasis suppressor molecule E-cadherin on prostate tumor cells. Cellular and molecular biology (Noisy-le-Grand, France). 2003 Nov;49(7):1009-1016.
Furbert-Harris, P. M. ; Parish-Gause, D. ; Hunter, K. A. ; Vaughn, T. R. ; Howland, C. ; Okomo-Awich, J. ; Forrest, K. ; Laniyan, I. ; Abdelnaby, A. ; Oredipe, O. A. / Activated eosinophils upregulate the metastasis suppressor molecule E-cadherin on prostate tumor cells. In: Cellular and molecular biology (Noisy-le-Grand, France). 2003 ; Vol. 49, No. 7. pp. 1009-1016.
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abstract = "Cell adhesion molecules (CAMs) play an important role in cancer metastasis by facilitating attachment to vascular endothelia, invasion and spread into secondary tissue sites. We have shown that activated eosinophils (EosA) inhibited the growth of prostate cancer (Pca) cells in vitro. In the present study, we examined the ability of EosA 24 hr conditioned supernatants (EosAcs) to modulate the expression of ICAM-1, VCAM-1, ELAM-1, E-cadherin and N-cadherin expression on human Pca cell lines, Du-145 and PC-3 by flow cytometry. TNF-alpha, IL-10 and IL-12 were also evaluated. ICAM-1, expressed on PC-3 and DU 145 cells, was enhanced by TNF-alpha and IL-10. ELAM-1 was present on DU 145 cells but absent on PC-3. TNF-alpha and IL-10 enhanced ELAM-1 on DU 145, but EosA 24 hr supematants failed to do so. All three cytokines, namely IL-10, IL-12 and TNF-alpha-induced ELAM-1 on PC-3 tumor cells. Although VCAM-1 was absent on DU 145 and PC-3 cells, it was expressed on DU-145 cells after exposure to EosA: tumor cell co-cultures, and was expressed on PC-3 following exposure to IL-10 and IL-12. N-cadherin and E-cadherin were both expressed on DU-145. While N-cadherin was expressed on PC-3 cells, E-cadherin was not. N-cadherin was enhanced on DU-145 and PC-3 cells following exposure to EosA co-culture and upregulated on PC-3 by IL-10 and EosA 24 hr supernatants, but decreased by IL-12. E-cadherin was up-regulated on DU 145 cells following co-culture with EosA and was induced on PC-3 by IL-10 and IL-12, but not by EosA co-culture and 24 hr supematants. In conclusion, inflammatory and non-inflammatory cytokines modulate CAM expression on Pca cells; EosA and EosA 24 hr supernatants also exerted modulatory activity of CAM expression. Most significantly, the metastasis suppressor molecule, E-cadherin was enhanced on DU 145 cells by EosA and induced on PC-3 by IL-10 and IL-12 both of which are produced by EosA. This suggests potential use of these cytokines in immunotherapeutic strategies for prostate cancer and its metastasis.",
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AU - Howland, C.

AU - Okomo-Awich, J.

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N2 - Cell adhesion molecules (CAMs) play an important role in cancer metastasis by facilitating attachment to vascular endothelia, invasion and spread into secondary tissue sites. We have shown that activated eosinophils (EosA) inhibited the growth of prostate cancer (Pca) cells in vitro. In the present study, we examined the ability of EosA 24 hr conditioned supernatants (EosAcs) to modulate the expression of ICAM-1, VCAM-1, ELAM-1, E-cadherin and N-cadherin expression on human Pca cell lines, Du-145 and PC-3 by flow cytometry. TNF-alpha, IL-10 and IL-12 were also evaluated. ICAM-1, expressed on PC-3 and DU 145 cells, was enhanced by TNF-alpha and IL-10. ELAM-1 was present on DU 145 cells but absent on PC-3. TNF-alpha and IL-10 enhanced ELAM-1 on DU 145, but EosA 24 hr supematants failed to do so. All three cytokines, namely IL-10, IL-12 and TNF-alpha-induced ELAM-1 on PC-3 tumor cells. Although VCAM-1 was absent on DU 145 and PC-3 cells, it was expressed on DU-145 cells after exposure to EosA: tumor cell co-cultures, and was expressed on PC-3 following exposure to IL-10 and IL-12. N-cadherin and E-cadherin were both expressed on DU-145. While N-cadherin was expressed on PC-3 cells, E-cadherin was not. N-cadherin was enhanced on DU-145 and PC-3 cells following exposure to EosA co-culture and upregulated on PC-3 by IL-10 and EosA 24 hr supernatants, but decreased by IL-12. E-cadherin was up-regulated on DU 145 cells following co-culture with EosA and was induced on PC-3 by IL-10 and IL-12, but not by EosA co-culture and 24 hr supematants. In conclusion, inflammatory and non-inflammatory cytokines modulate CAM expression on Pca cells; EosA and EosA 24 hr supernatants also exerted modulatory activity of CAM expression. Most significantly, the metastasis suppressor molecule, E-cadherin was enhanced on DU 145 cells by EosA and induced on PC-3 by IL-10 and IL-12 both of which are produced by EosA. This suggests potential use of these cytokines in immunotherapeutic strategies for prostate cancer and its metastasis.

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