Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen

Nusrettin Ulker, Keith D. Lewis, Leroy E. Hood, Iwona Stroynowski

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Among the best characterized non-classical mouse major histocompatibility antigens are the Qa-2 molecules. These proteins can serve as targets for allogenic cytotoxic T cells and as signal transducing molecules. They are structurally similar to H-2 transplantation antigens in their Ntarminal and β2-microglobulin binding domains but differ at their C-termini. While the H-2 antigens span the cell membrane, the Qa-2 molecules are attached to the cell surface via phospholipid anchors. The genetic information encoding this attachment is contained in exon 5. In concanavalin A activated splenocytes the expression of membrane bound Qa-2 antigens declines and, simultaneously, soluble forms of Qa-2 molecules are secreted. We demonstrate here that the soluble Qa-2 polypeptides are translated from alternatively spliced mRNAs lacking exon 5, while the membrane forms are encoded by the full-size transcripts. In cultured cells the alternative splicing of the Qa-2 message is induced by Tcell activation with concanavalin A. The canonical mRNA encoding the membrane form of Qa-2 predominates in unstimulated mouse tissues but the cultured cell lines, like activated T cells, express enhanced levels of the truncated mRNA. In some cell lines almost all Qa-2 transcripts lack exon 5. For example, in L cells, mRNAs encoding soluble Qa-2 molecules are at least 10 times more abundant than Qa-2 transcripts encoding phospholipid anchored antigens. These findings are discussed in terms of potentlal functions of membrane bound and secreted Qa-2 molecules.

Original languageEnglish (US)
Pages (from-to)3839-3847
Number of pages9
JournalEMBO Journal
Volume9
Issue number12
StatePublished - 1990

Fingerprint

T-cells
H-2 Antigens
T-Lymphocytes
Exons
Messenger RNA
Histocompatibility Antigens
Molecules
Membranes
Concanavalin A
Cultured Cells
Phospholipids
Cells
Cell Line
Alternative Splicing
Cell Membrane
Cell membranes
Anchors
Antigens
Peptides
Q surface antigens

Keywords

  • Activated T cells
  • Alternative splicing
  • Major histocompatibility complex
  • Qa-2 molecules

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen. / Ulker, Nusrettin; Lewis, Keith D.; Hood, Leroy E.; Stroynowski, Iwona.

In: EMBO Journal, Vol. 9, No. 12, 1990, p. 3839-3847.

Research output: Contribution to journalArticle

Ulker, Nusrettin ; Lewis, Keith D. ; Hood, Leroy E. ; Stroynowski, Iwona. / Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen. In: EMBO Journal. 1990 ; Vol. 9, No. 12. pp. 3839-3847.
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