Activation-dependent surface expression of LOX-1 in human platelets

Mingyi Chen, Makoto Kakutani, Tomoh Masaki, Tatsuya Sawamura, Mingyi Chen, Shuh Narumiya, Takahiko Naruko, Makiko Ueda, Tatsuya Sawamura

Research output: Contribution to journalArticle

115 Scopus citations

Abstract

Lectin-like oxidized LDL receptor-1 (LOX-1) was initially identified as an oxidized LDL receptor in aortic endothelial cells. Here we identified LOX-1 mRNA and protein in human platelets in addition to recent findings on the expression in macrophages and smooth muscle cells. The presence of LOX-1 was further confirmed in the megakaryocytic cell lines. Flow cytometric analyses revealed that LOX-1 was exposed on the surface of platelets in an activation-dependent manner. Consistently, the activation-dependent binding of OxLDL to platelets was mostly inhibited by anti-LOX-1 antibody. Immunohistochemistry of the atherosclerotic plaque from a patient with unstable angina pectoris (UAP) revealed accumulation of LOX-1 protein at the site of thrombus. As LOX-1 recognizes and binds activated platelets, exposure of LOX-1 on activated platelets surface might assist thrombosis formation.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number1
DOIs
StatePublished - 2001

Keywords

  • Human platelet
  • LOX-1
  • Oxidized LDL

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Chen, M., Kakutani, M., Masaki, T., Sawamura, T., Chen, M., Narumiya, S., Naruko, T., Ueda, M., & Sawamura, T. (2001). Activation-dependent surface expression of LOX-1 in human platelets. Biochemical and Biophysical Research Communications, 282(1), 153-158. https://doi.org/10.1006/bbrc.2001.4516