Activation of antigen-enriched B cells. II. Role of linked recognition in B cell proliferation to thymus-dependent antigens

E. C. Snow, R. J. Noelle, J. W. Uhr, E. S. Vitetta

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Abstract

The responsiveness to T-dependent (TD) and T-independent (TI) TNP-antigens of murine splenic B cells previously enriched for antigen-binding cells (ABC) was examined. TNP-TI antigens induced B cell proliferation. TNP-TD antigens did not induce a proliferative response regardless of the physical form or nature of the TNP-TD antigen (e.g., soluble vs particulate, low or high haptenation of carrier, TNP on various insoluble matrices, etc.). TNP-TD antigens were effective in enhancing the response of the TNP-ABC to all concentrations of lipopolysaccharide (LPS) tested, indicating that binding of antigen to surface immunoglobulin alters the LPS responsiveness of the cell. Irradiated, keyhole limpet hemocyanin- (KLH) primed T cells induced a threefold to fourfold greater B cell proliferative response with TNP-KLH than with fluoresceinated KLH (FLU-KLH) or FLU-KLH together with TNP-human serum albumin (TNP-HSA). Therefore, linked recognition appears essential for optimal T cell-mediated B cell proliferation, whereas the induction of B cell proliferation via nonlinked, carrier-activated T cells is a minor component of the response.

Original languageEnglish (US)
Pages (from-to)614-618
Number of pages5
JournalJournal of Immunology
Volume130
Issue number2
StatePublished - 1983

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CD80 Antigens
Thymus Gland
B-Lymphocytes
Cell Proliferation
Viral Tumor Antigens
Antigens
T Independent Antigens
T-Lymphocytes
Lipopolysaccharides
B-Cell Antigen Receptors
Serum Albumin
keyhole-limpet hemocyanin

ASJC Scopus subject areas

  • Immunology

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Activation of antigen-enriched B cells. II. Role of linked recognition in B cell proliferation to thymus-dependent antigens. / Snow, E. C.; Noelle, R. J.; Uhr, J. W.; Vitetta, E. S.

In: Journal of Immunology, Vol. 130, No. 2, 1983, p. 614-618.

Research output: Contribution to journalArticle

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AU - Noelle, R. J.

AU - Uhr, J. W.

AU - Vitetta, E. S.

PY - 1983

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AB - The responsiveness to T-dependent (TD) and T-independent (TI) TNP-antigens of murine splenic B cells previously enriched for antigen-binding cells (ABC) was examined. TNP-TI antigens induced B cell proliferation. TNP-TD antigens did not induce a proliferative response regardless of the physical form or nature of the TNP-TD antigen (e.g., soluble vs particulate, low or high haptenation of carrier, TNP on various insoluble matrices, etc.). TNP-TD antigens were effective in enhancing the response of the TNP-ABC to all concentrations of lipopolysaccharide (LPS) tested, indicating that binding of antigen to surface immunoglobulin alters the LPS responsiveness of the cell. Irradiated, keyhole limpet hemocyanin- (KLH) primed T cells induced a threefold to fourfold greater B cell proliferative response with TNP-KLH than with fluoresceinated KLH (FLU-KLH) or FLU-KLH together with TNP-human serum albumin (TNP-HSA). Therefore, linked recognition appears essential for optimal T cell-mediated B cell proliferation, whereas the induction of B cell proliferation via nonlinked, carrier-activated T cells is a minor component of the response.

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