Activation of antigen-specific B cells: role of T cells, cytokines, and antigen in induction of growth and differentiation

R. J. Noelle, E. C. Snow, J. W. Uhr, E. S. Vitetta

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Abstract

T cells and cytokines were used to activate highly enriched populations of 2,4,6-trinitrophenyl (TNP)-binding B cells (TNP-ABC). TNP-ABC did not proliferate or differentiate when they were cultured with thymus-dependent (TD) antigen, even in the presence of supernatants known to contain B-cell growth and differentiation factors. However, purified TNP-ABC did proliferate and differentiate when they were cultured with TD antigen in the presence of carrier-primed T cells and antigen (TNP-keyhole limpet hemocyanin) i.e., linked recognition. TNP-ABC blasts generated under conditions of linked recognition proliferated and differentiated in response to cytokines in the absence of T cells and antigen. In contrast, under conditions of nonlinked recognition (hapten and carrier on different molecules) TNP-ABC blasts also proliferated but did not differentiate in response to the same cytokines. These results indicate that antigen-specific 'resting' B cells must be activated by T cells and antigen prior to becoming response to cytokines. Furthermore, activation under conditions of linked and nonlinked recognition generates two different types of blasts with regard to their subsequent response to cytokines.

Original languageEnglish (US)
Pages (from-to)6628-6631
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number21 I
StatePublished - 1983

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CD80 Antigens
Viral Tumor Antigens
Cytokines
T-Lymphocytes
Growth
Antigens
Thymus Gland
B-Lymphocytes
Growth Differentiation Factors
Haptens
Interleukin-6
Population

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Activation of antigen-specific B cells: role of T cells, cytokines, and antigen in induction of growth and differentiation",
abstract = "T cells and cytokines were used to activate highly enriched populations of 2,4,6-trinitrophenyl (TNP)-binding B cells (TNP-ABC). TNP-ABC did not proliferate or differentiate when they were cultured with thymus-dependent (TD) antigen, even in the presence of supernatants known to contain B-cell growth and differentiation factors. However, purified TNP-ABC did proliferate and differentiate when they were cultured with TD antigen in the presence of carrier-primed T cells and antigen (TNP-keyhole limpet hemocyanin) i.e., linked recognition. TNP-ABC blasts generated under conditions of linked recognition proliferated and differentiated in response to cytokines in the absence of T cells and antigen. In contrast, under conditions of nonlinked recognition (hapten and carrier on different molecules) TNP-ABC blasts also proliferated but did not differentiate in response to the same cytokines. These results indicate that antigen-specific 'resting' B cells must be activated by T cells and antigen prior to becoming response to cytokines. Furthermore, activation under conditions of linked and nonlinked recognition generates two different types of blasts with regard to their subsequent response to cytokines.",
author = "Noelle, {R. J.} and Snow, {E. C.} and Uhr, {J. W.} and Vitetta, {E. S.}",
year = "1983",
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pages = "6628--6631",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
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TY - JOUR

T1 - Activation of antigen-specific B cells

T2 - role of T cells, cytokines, and antigen in induction of growth and differentiation

AU - Noelle, R. J.

AU - Snow, E. C.

AU - Uhr, J. W.

AU - Vitetta, E. S.

PY - 1983

Y1 - 1983

N2 - T cells and cytokines were used to activate highly enriched populations of 2,4,6-trinitrophenyl (TNP)-binding B cells (TNP-ABC). TNP-ABC did not proliferate or differentiate when they were cultured with thymus-dependent (TD) antigen, even in the presence of supernatants known to contain B-cell growth and differentiation factors. However, purified TNP-ABC did proliferate and differentiate when they were cultured with TD antigen in the presence of carrier-primed T cells and antigen (TNP-keyhole limpet hemocyanin) i.e., linked recognition. TNP-ABC blasts generated under conditions of linked recognition proliferated and differentiated in response to cytokines in the absence of T cells and antigen. In contrast, under conditions of nonlinked recognition (hapten and carrier on different molecules) TNP-ABC blasts also proliferated but did not differentiate in response to the same cytokines. These results indicate that antigen-specific 'resting' B cells must be activated by T cells and antigen prior to becoming response to cytokines. Furthermore, activation under conditions of linked and nonlinked recognition generates two different types of blasts with regard to their subsequent response to cytokines.

AB - T cells and cytokines were used to activate highly enriched populations of 2,4,6-trinitrophenyl (TNP)-binding B cells (TNP-ABC). TNP-ABC did not proliferate or differentiate when they were cultured with thymus-dependent (TD) antigen, even in the presence of supernatants known to contain B-cell growth and differentiation factors. However, purified TNP-ABC did proliferate and differentiate when they were cultured with TD antigen in the presence of carrier-primed T cells and antigen (TNP-keyhole limpet hemocyanin) i.e., linked recognition. TNP-ABC blasts generated under conditions of linked recognition proliferated and differentiated in response to cytokines in the absence of T cells and antigen. In contrast, under conditions of nonlinked recognition (hapten and carrier on different molecules) TNP-ABC blasts also proliferated but did not differentiate in response to the same cytokines. These results indicate that antigen-specific 'resting' B cells must be activated by T cells and antigen prior to becoming response to cytokines. Furthermore, activation under conditions of linked and nonlinked recognition generates two different types of blasts with regard to their subsequent response to cytokines.

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