Activation of dynamin II by POPC in giant unilamellar vesicles: A two-photon fluorescence microscopy study

L. A. Bagatolli, D. D. Binns, D. M. Jameson, Joseph P. Albanesi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The interaction of dynamin II with giant unilamellar vesicles was studied using two-photon fluorescence microscopy. Dynamin II, labeled with fluorescein, was injected into a microscope chamber containing giant unilamellar vesicles, which were composed of either pure 1-palmitoyl-2-oleoyl-snglycero-3-phosphocholine (POPC) or a mixture of POPC and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Binding of the fluorescent dynamin II to giant unilamellar vesicles, in the presence and absence of PI(4,5)P2, was directly observed using two-photon fluorescence microscopy. This binding was also visualized using the fluorescent N-methylanthraniloyl guanosine 5′-[γ-thio]triphosphate analogue. The membrane probe 6-dodecanoyl-2-dimethylamine-naphthalene was used to monitor the physical state of the lipid in the giant unilamellar vesicles in the absence and presence of dynamin. A surprising finding was the fact that dynamin II bound to vesicles in the absence of PI(4,5)P2. Activation of the GTPase activity of dynamin II by pure POPC was then shown.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalJournal of Protein Chemistry
Volume21
Issue number6
DOIs
StatePublished - Aug 2002

Fingerprint

Dynamin II
Unilamellar Liposomes
Phosphorylcholine
Fluorescence microscopy
Photons
Fluorescence Microscopy
Chemical activation
Naphthalene
Lipids
Microscopes
Membranes
Dynamins
Guanosine
GTP Phosphohydrolases
Phosphatidylinositols
Fluorescein

Keywords

  • 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)
  • Dynamin
  • Fluorescence
  • Giant unilamellar vesicles
  • N-methylanthraniloyl guanosine 5′-[γ-thio]triphosphate (mant-GTPγS)
  • Two-photon microscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Activation of dynamin II by POPC in giant unilamellar vesicles : A two-photon fluorescence microscopy study. / Bagatolli, L. A.; Binns, D. D.; Jameson, D. M.; Albanesi, Joseph P.

In: Journal of Protein Chemistry, Vol. 21, No. 6, 08.2002, p. 383-391.

Research output: Contribution to journalArticle

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