Activation of mitogen-activated protein kinase cascades by p21-activated protein kinases in cell-free extracts of Xenopus oocytes

Anthony Polverino, Jeff Frost, Peirong Yang, Michele Hutchison, Aaron M. Neiman, Melanie H. Cobb, Stevan Marcus

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Abstract

In the evolutionarily distant yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, genetic evidence suggests that activation of pheromone-induced mitogen-activated protein kinase (MAPK) cascades involves the function of the p21(cdc42/rac1)-activated protein kinases (PAKs) Ste20 and Shk1, respectively. In this report, we show that purified Ste20 and Shk1 were each capable of inducing p42(MAPK) activation in cell-free extracts of Xenopus laevis oocytes, while a mammalian Ste20/Shk1-related protein kinase, p65(pak) (Pak1), did not induce activation of p42(MAPK). In contrast to p42(MAPK), activation of JNK/SAPK in Xenopus oocyte extracts was induced by both the yeast Ste20 and Shk1 kinases, as well as by mammalian Pak1. Our results demonstrate that MAPK cascades that are responsive to PAKs are conserved in higher eukaryotes and suggest that distinct PAKs may regulate distinct MAPK modules.

Original languageEnglish (US)
Pages (from-to)26067-26070
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number44
DOIs
StatePublished - Nov 3 1995

Fingerprint

p21-Activated Kinases
Mitogen-Activated Protein Kinase 1
Xenopus
Mitogen-Activated Protein Kinases
Cell Extracts
Protein Kinases
Oocytes
Chemical activation
Yeast
rac1 GTP-Binding Protein
cdc42 GTP-Binding Protein
Yeasts
Schizosaccharomyces
Pheromones
Xenopus laevis
Eukaryota
Saccharomyces cerevisiae
Phosphotransferases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Activation of mitogen-activated protein kinase cascades by p21-activated protein kinases in cell-free extracts of Xenopus oocytes. / Polverino, Anthony; Frost, Jeff; Yang, Peirong; Hutchison, Michele; Neiman, Aaron M.; Cobb, Melanie H.; Marcus, Stevan.

In: Journal of Biological Chemistry, Vol. 270, No. 44, 03.11.1995, p. 26067-26070.

Research output: Contribution to journalArticle

Polverino, Anthony ; Frost, Jeff ; Yang, Peirong ; Hutchison, Michele ; Neiman, Aaron M. ; Cobb, Melanie H. ; Marcus, Stevan. / Activation of mitogen-activated protein kinase cascades by p21-activated protein kinases in cell-free extracts of Xenopus oocytes. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 44. pp. 26067-26070.
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