Activation of murine B cells. II. Dextran sulfate removes the requirement for cellular interaction during lipopolysaccharide-induced mitogenesis

Gayle D. Wetzel, John R. Kettman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Analysis of murine spleen cell growth at various initial cell densities suggested a requirement for cell interaction in some lipopolysaccharide (LPS)-induced B-cell proliferation. B-Cell mitogenesis by LPS did not exhibit single-hit kinetics. In addition, the proportion of cells demonstrating increase in cell size and transcriptional activity induced by LPS alone decreased with decreasing cell density. Dextran sulfate (DXS), a B-cell mitogen also described as requiring accessory cells for its mitogenic activity, was found to act synergistically with LPS in the induction of B-cell proliferation. Furthermore, the addition of DXS to LPS removed requirements for cell interaction in B-cell mitogenesis. As a consequence, the proportion of cells activated by the combination of LPS and DXS did not decrease as the initial cell density decreased.

Original languageEnglish (US)
Pages (from-to)176-189
Number of pages14
JournalCellular Immunology
Volume61
Issue number1
DOIs
StatePublished - 1981

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Dextran Sulfate
Lipopolysaccharides
B-Lymphocytes
Cell Count
Cell Communication
Cell Proliferation
Cell Size
Mitogens
Spleen
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Activation of murine B cells. II. Dextran sulfate removes the requirement for cellular interaction during lipopolysaccharide-induced mitogenesis. / Wetzel, Gayle D.; Kettman, John R.

In: Cellular Immunology, Vol. 61, No. 1, 1981, p. 176-189.

Research output: Contribution to journalArticle

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