The effect of phosphoinositides on the activity of protein kinase C (PKC) isotypes was investigated. PKC α, βI, βII, γ, δ, ε, η, and ζ were expressed in baculovirus-infected insect cells and purified by column chromatography. The calcium-activated PKC isotypes α, βI, βII, and γ were not significantly activated by any of the phosphoinositides investigated (phosphatidylinositol-4-phosphate (PtdIns-4-P), PtdIns-3-P, PtdIns-4,5-P2, PtdIns-3,4-P2, and PtdIns-3,4,5-P3) when added in the presence of concentrations of phosphatidylserine that give maximal stimulation. The calcium-insensitive PKC isotypes δ, ε, and η also showed little response to PtdIns-3-P, PtdIns-4-P, or PtdIns-4,5-P2 when these lipids were added in the presence of phosphatidylserine. In contrast, PtdIns-3,4-P2 and PtdIns- 3,4,5-P3 caused a 5-15-fold stimulation of these enzymes compared with phosphatidylserine alone. 50% maximal stimulation of PKC ε by PtdIns-3,4,5- P3 occurred when this lipid was present at about 1% of the carrier PtdIns- 4,5-P2 (about 100 nM). These lipids had little effect on baculovirus- expressed PKC ζ, which was constitutively active. A short chain version of PtdIns-3,4,5-P3, dioctanoyl-PtdIns-3,4,5-P3, activated PKC δ, ε, and η in the absence of other lipids, whereas a short chain version of PtdIns-4,5- P2, dihexanoyl-PtdIns-4,5-P2, did not. Since PtdIns-3,4-P2 and PtdIns- 3,4,5-P3 are nominally absent in unstimulated cells and appear within seconds to minutes of stimulation by various cell activators, these lipids could act as second messengers to activate PKC δ, ε, or η in vivo.
|Original language||English (US)|
|Number of pages||10|
|Journal||Journal of Biological Chemistry|
|State||Published - 1994|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology