Abstract
By using both pulse labeling of nascent RNA chains and lactoperoxidase-catalyzed cell surface radioiodination, we examined both the de novo synthesis of mRNA for γ-chains and the expression of membrane IgG (mIgG) on cells which had been stimulated with LPS plus a T cell supernatant (SN) containing a B cell differentiation factor for IgG1 (BCDFγ). Our results show that neither nascent mRNA for γ1 chains nor mIgG1 can be detected in B lymphocytes until they have been stimulated by both LPS and BCDFγ-containing T cell SN, and suggest that cell surface expression and secretion of IgG1 are coordinately controlled.
Original language | English (US) |
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Pages (from-to) | 1465-1469 |
Number of pages | 5 |
Journal | Journal of Immunology |
Volume | 135 |
Issue number | 2 |
State | Published - 1985 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology