Activation of the γ1 gene by lipopolysaccharide and T cell-derived lymphokines containing a B cell differentiation factor for IgG1 (BCDFγ)

D. Yuan, E. A. Weiss, J. E. Layton, P. H. Krammer, E. S. Vitetta

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

By using both pulse labeling of nascent RNA chains and lactoperoxidase-catalyzed cell surface radioiodination, we examined both the de novo synthesis of mRNA for γ-chains and the expression of membrane IgG (mIgG) on cells which had been stimulated with LPS plus a T cell supernatant (SN) containing a B cell differentiation factor for IgG1 (BCDFγ). Our results show that neither nascent mRNA for γ1 chains nor mIgG1 can be detected in B lymphocytes until they have been stimulated by both LPS and BCDFγ-containing T cell SN, and suggest that cell surface expression and secretion of IgG1 are coordinately controlled.

Original languageEnglish (US)
Pages (from-to)1465-1469
Number of pages5
JournalJournal of Immunology
Volume135
Issue number2
StatePublished - Dec 1 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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