TY - JOUR
T1 - Activation of the reaper gene during ectopic cell killing in Drosophila
AU - Nordstrom, William
AU - Chen, Po
AU - Steller, Hermann
AU - Abrams, John M.
N1 - Funding Information:
We thank Paul Friesen for providing p35 cDNA and antisera, Lois Miller for a p35 vector, Q. Chu and C. Doe for hkb DNA, Lucy Cherbas for actin±β-gal DNA, Jonson Varkey, Pauline Lee, Kim Plunk, and Tien Thach for excellent technical assistance, Lisa Johnson for help with ¯ow cytometry, Helmut Kramer for the HA-hook plasmid, Mark Walberg for HA-tagged rpr DNA, Jerry Shay, Richard Anderson, George Bloom, and Fred Grinnell for helpful discussions. H.S. is an associate investigator with the Howard Hughes Medical Institute. This work was supported by grants to J.M.A. from the National Institute of Health (AG12466) and from the Texas Advanced Technology Program (003660061).
PY - 1996/11/25
Y1 - 1996/11/25
N2 - The product of the reaper (rpr) gene is required for programmed cell death in Drosophila. We examined rpr expression during ectopic cell aths caused by ionizing radiation or aberrant development. In both instances, dramatic induction of rpr expression was observed. A genomic fragment upstream of rpr confers this regulatory behavior upon a lacZ reporter transgene. In a model cell culture system, conditional expression of REAPER is sufficient to induce massive apoptosis that can be prevented by the anti-apoptotic protein p35. Overall, these results suggest that diverse signals converge at, or upstream of, rpr-associated transcriptional regulatory elements that can function to initiate a common apoptotic pathway involving ICE-like protease activity.
AB - The product of the reaper (rpr) gene is required for programmed cell death in Drosophila. We examined rpr expression during ectopic cell aths caused by ionizing radiation or aberrant development. In both instances, dramatic induction of rpr expression was observed. A genomic fragment upstream of rpr confers this regulatory behavior upon a lacZ reporter transgene. In a model cell culture system, conditional expression of REAPER is sufficient to induce massive apoptosis that can be prevented by the anti-apoptotic protein p35. Overall, these results suggest that diverse signals converge at, or upstream of, rpr-associated transcriptional regulatory elements that can function to initiate a common apoptotic pathway involving ICE-like protease activity.
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U2 - 10.1006/dbio.1996.0296
DO - 10.1006/dbio.1996.0296
M3 - Article
C2 - 8948586
AN - SCOPUS:0030602011
SN - 0012-1606
VL - 180
SP - 213
EP - 226
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -