Activity and regulation of the centrosome-associated proteasome

Rosalind P. Fabunmi, W. Christian Wigley, Philip J. Thomas, George N. DeMartino

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Regulated proteolysis is important for maintaining appropriate cellular levels of many proteins. The bulk of intracellular protein degradation is catalyzed by the proteasome. Recently, the centrosome was identified as a novel site for concentration of the proteasome and associated regulatory proteins (Wigley, W. C., Fabunmi, R. P., Lee, M. G., Marino, C. R., Muallem, S., DeMartino, G. N., and Thomas, P. J. (1999) J. Cell Biol. 145, 481-490). Here we provide evidence that centrosomes contain the active 26 S proteasome that degrades ubiquitinated-protein and proteasome-specific peptide substrates. Moreover, the centrosomes contain an ubiquitin isopeptidase activity. The proteolytic activity is ATP-dependent and is inhibited by proteasome inhibitors. Notably, treatment of cells with inhibitors of proteasome activity promotes redistribution of the proteasome and associated regulatory proteins to the centrosome independent of an intact microtubule system. These data provide biochemical evidence for active proteasomal complexes at the centrosome, highlighting a novel function for this organizing structure.

Original languageEnglish (US)
Pages (from-to)409-413
Number of pages5
JournalJournal of Biological Chemistry
Volume275
Issue number1
DOIs
StatePublished - Jan 7 2000

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Centrosome
Proteasome Endopeptidase Complex
Proteasome Inhibitors
Proteolysis
Proteins
Ubiquitinated Proteins
Microtubules
Adenosine Triphosphate
Cells
Degradation
Peptides
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Activity and regulation of the centrosome-associated proteasome. / Fabunmi, Rosalind P.; Wigley, W. Christian; Thomas, Philip J.; DeMartino, George N.

In: Journal of Biological Chemistry, Vol. 275, No. 1, 07.01.2000, p. 409-413.

Research output: Contribution to journalArticle

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