Aktivnost' lipoksigenazy, vkliuchennoǐ v obrashchennye mitselly aérozolia OT v oktane.

Translated title of the contribution: Activity of lipoxygenase incorporated into reversed micelles of aerosol OT in octane

I. A. Butovich, T. V. Mogilevich, V. P. Kukhar'

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Soybean lipoxygenase (EC 1.13.11.12) incorporated into the reversed micelles of aerosol OT in octane has been studied for its catalytic properties. The enzyme is shown to preserve up to 10% activity as compared with the activity in the aqueous solution. In this case Km of lipoxygenase for linoleic acid increases from 10(-5) M to 5 X 10(-4) M. The activity of lipoxygenase is maximal, the aerosol OT concentration being 0.03 M and a degree of reversed micelle hydratation 40. Cationic detergents of the cetyltrimethyl ammonium bromide type are not good to form reversed micelles of lipoxygenase, since they inhibit the latter with IC50 = (4 divided by 6) x 10(-4) M. The lipoxygenase preparations in reversed micelles of aerosol OT in octane may be used to synthesize natural metabolites of polyunsaturated fatty acids, for instance of eicosanoids.

Original languageUndefined
Pages (from-to)54-59
Number of pages6
JournalUkrainskii biokhimicheskii zhurnal
Volume61
Issue number2
Publication statusPublished - Mar 1989

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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