Aktivnost' lipoksigenazy, vkliuchennoǐ v obrashchennye mitselly aérozolia OT v oktane.

I. A. Butovich; T. V. Mogilevich; V. P. Kukhar'

Aktivnost' lipoksigenazy, vkliuchennoǐ v obrashchennye mitselly aérozolia OT v oktane.

Translated title of the contribution: Activity of lipoxygenase incorporated into reversed micelles of aerosol OT in octane

I. A. Butovich, T. V. Mogilevich, V. P. Kukhar'

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Soybean lipoxygenase (EC 1.13.11.12) incorporated into the reversed micelles of aerosol OT in octane has been studied for its catalytic properties. The enzyme is shown to preserve up to 10% activity as compared with the activity in the aqueous solution. In this case Km of lipoxygenase for linoleic acid increases from 10(-5) M to 5 X 10(-4) M. The activity of lipoxygenase is maximal, the aerosol OT concentration being 0.03 M and a degree of reversed micelle hydratation 40. Cationic detergents of the cetyltrimethyl ammonium bromide type are not good to form reversed micelles of lipoxygenase, since they inhibit the latter with IC50 = (4 divided by 6) x 10(-4) M. The lipoxygenase preparations in reversed micelles of aerosol OT in octane may be used to synthesize natural metabolites of polyunsaturated fatty acids, for instance of eicosanoids.

Translated title of the contribution	Activity of lipoxygenase incorporated into reversed micelles of aerosol OT in octane
Original language	Russian
Pages (from-to)	54-59
Number of pages	6
Journal	Ukrainskii biokhimicheskii zhurnal
Volume	61
Issue number	2
State	Published - Mar 1989

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Aktivnost' lipoksigenazy, vkliuchennoǐ v obrashchennye mitselly a{\'e}rozolia OT v oktane.",

abstract = "Soybean lipoxygenase (EC 1.13.11.12) incorporated into the reversed micelles of aerosol OT in octane has been studied for its catalytic properties. The enzyme is shown to preserve up to 10% activity as compared with the activity in the aqueous solution. In this case Km of lipoxygenase for linoleic acid increases from 10(-5) M to 5 X 10(-4) M. The activity of lipoxygenase is maximal, the aerosol OT concentration being 0.03 M and a degree of reversed micelle hydratation 40. Cationic detergents of the cetyltrimethyl ammonium bromide type are not good to form reversed micelles of lipoxygenase, since they inhibit the latter with IC50 = (4 divided by 6) x 10(-4) M. The lipoxygenase preparations in reversed micelles of aerosol OT in octane may be used to synthesize natural metabolites of polyunsaturated fatty acids, for instance of eicosanoids.",

author = "Butovich, {I. A.} and Mogilevich, {T. V.} and Kukhar', {V. P.}",

year = "1989",

month = mar,

language = "Russian",

volume = "61",

pages = "54--59",

journal = "Ukrainskii biokhimicheskii zhurnal",

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publisher = "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU)",

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T1 - Aktivnost' lipoksigenazy, vkliuchennoǐ v obrashchennye mitselly aérozolia OT v oktane.

AU - Butovich, I. A.

AU - Mogilevich, T. V.

AU - Kukhar', V. P.

PY - 1989/3

Y1 - 1989/3

N2 - Soybean lipoxygenase (EC 1.13.11.12) incorporated into the reversed micelles of aerosol OT in octane has been studied for its catalytic properties. The enzyme is shown to preserve up to 10% activity as compared with the activity in the aqueous solution. In this case Km of lipoxygenase for linoleic acid increases from 10(-5) M to 5 X 10(-4) M. The activity of lipoxygenase is maximal, the aerosol OT concentration being 0.03 M and a degree of reversed micelle hydratation 40. Cationic detergents of the cetyltrimethyl ammonium bromide type are not good to form reversed micelles of lipoxygenase, since they inhibit the latter with IC50 = (4 divided by 6) x 10(-4) M. The lipoxygenase preparations in reversed micelles of aerosol OT in octane may be used to synthesize natural metabolites of polyunsaturated fatty acids, for instance of eicosanoids.

AB - Soybean lipoxygenase (EC 1.13.11.12) incorporated into the reversed micelles of aerosol OT in octane has been studied for its catalytic properties. The enzyme is shown to preserve up to 10% activity as compared with the activity in the aqueous solution. In this case Km of lipoxygenase for linoleic acid increases from 10(-5) M to 5 X 10(-4) M. The activity of lipoxygenase is maximal, the aerosol OT concentration being 0.03 M and a degree of reversed micelle hydratation 40. Cationic detergents of the cetyltrimethyl ammonium bromide type are not good to form reversed micelles of lipoxygenase, since they inhibit the latter with IC50 = (4 divided by 6) x 10(-4) M. The lipoxygenase preparations in reversed micelles of aerosol OT in octane may be used to synthesize natural metabolites of polyunsaturated fatty acids, for instance of eicosanoids.

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Aktivnost' lipoksigenazy, vkliuchennoǐ v obrashchennye mitselly aérozolia OT v oktane.

Abstract

ASJC Scopus subject areas

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