Activity of the basolateral K+ channels is coupled to the Na+-K+-ATPase in the cortical collecting duct

Shigeaki Muto, Yasushi Asano, WenHui Wang, Donald Seldin, Gerhard Giebisch

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12 Scopus citations

Abstract

Microelectrode and patch-clamp techniques were used in the isolated cortical collecting duct to study the effects of stimulating Na +-K+-ATPase by raising bath K+ (Fujii Y and Katz AI. Am J Physiol Renal Fluid Electrolyte Physiol 257: F595-F601, 1989 and Muto S, Asano Y, Seldin D, and Giebisch. Am J Physiol Renal Physiol 276: F143-F158, 1999) on the transepithelial (VT) and basolateral membrane (VB) voltages and basolateral K+ channel activity. Increasing bath K+ from 2.5 to 8.5 mM resulted in an initial hyperpolarization of both VT and VB followed by a delayed depolarization. The effects of raising bath K+ on V T and VB were attenuated by decreasing luminal Na + from 146.8 to 14.0 mM and were abolished by removal of luminal Na+, whereas those were magnified in desoxycorticosterone acetate (DOCA)-treated rabbits. Increasing bath K+ also led to a significant reduction of the intracellular Na+ and Ca2+ concentrations. The transepithelial conductance (GT) or fractional apical membrane resistance (fRA) were unaltered during the initial hyperpolarization phase, whereas, in the late depolarization phase, there were an increase in GT and a decrease in fRA, both of which were attenuated in the presence of low luminal Na+ (14.0 mM). In tubules from DOCA-treated animals, bath Ba2+ not only caused a significantly larger initial hyperpolarization of VT and V B but also blunted the late depolarization by high bath K +. Nω-nitro-L-arginine methyl ester (L-NAME) partially mimicked the effect of Ba2+ and decreased the amplitude of the late depolarization. Patch-clamp experiments showed that raising bath K+ from 2.5 to 8.5 mM resulted in an increased activity of the basolateral K + channel, which was absent in the presence of L-NAME. We conclude that stimulation of Na+-K+-ATPase increases the basolateral K+ conductance and that this effect involves suppression of nitric oxide-dependent inhibition of K+ channels.

Original languageEnglish (US)
Pages (from-to)F945-F954
JournalAmerican Journal of Physiology - Renal Physiology
Volume285
Issue number5 54-5
StatePublished - Nov 1 2003

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Keywords

  • Basolateral potassium channels
  • Nitric oxide
  • Potassium secretion
  • Sodium transport

ASJC Scopus subject areas

  • Physiology
  • Urology

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