Acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus strains maintain an antibacterial type VI secretion system with versatile effector repertoires

Peng Li, Lisa N. Kinch, Ann Ray, Ankur B. Dalia, Qian Cong, Linda M. Nunan, Andrew Camilli, Nick V. Grishin, Dor Salomon, Kim Orth

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. AHPND is caused by toxic strains of Vibrio parahaemolyticus that have acquired a "selfish plasmid" encoding the deadly binary toxins PirAvp/PirBvp. To better understand the repertoire of virulence factors in AHPND-causing V. parahaemolyticus, we conducted a comparative analysis using the genome sequences of the clinical strain RIMD2210633 and of environmental non-AHPND and toxic AHPND isolates of V. parahaemolyticus. Interestingly, we found that all of the AHPND strains, but none of the non-AHPND strains, harbor the antibacterial type VI secretion system 1 (T6SS1), which we previously identified and characterized in the clinical isolate RIMD2210633. This finding suggests that the acquisition of this T6SS might confer to AHPND-causing V. parahaemolyticus a fitness advantage over competing bacteria and facilitate shrimp infection. Additionally, we found highly dynamic effector loci in the T6SS1 of AHPND-causing strains, leading to diverse effector repertoires. Our discovery provides novel insights into AHPND-causing pathogens and reveals a potential target for disease control.

Original languageEnglish (US)
Article numbere00737-17
JournalApplied and Environmental Microbiology
Volume83
Issue number13
DOIs
StatePublished - 2017

Fingerprint

Vibrio parahaemolyticus
secretion
necrosis
Necrosis
Poisons
shrimp
Type VI Secretion Systems
type VI secretion system
shrimp fisheries
disease control
Acute Disease
Virulence Factors
virulence
plasmid
toxin
plasmids
toxins
Industry
harbor
Plasmids

Keywords

  • AHPND
  • MIX effectors
  • Shrimp
  • T3SS
  • T6SS
  • Type III secretion system
  • Type VI secretion system
  • Vibrio
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus strains maintain an antibacterial type VI secretion system with versatile effector repertoires. / Li, Peng; Kinch, Lisa N.; Ray, Ann; Dalia, Ankur B.; Cong, Qian; Nunan, Linda M.; Camilli, Andrew; Grishin, Nick V.; Salomon, Dor; Orth, Kim.

In: Applied and Environmental Microbiology, Vol. 83, No. 13, e00737-17, 2017.

Research output: Contribution to journalArticle

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