Acyl-homoserine-lactone autoinducer in the gastrointesinal tract of feedlot cattle and correlation to season, E. Coli O157: H7 prevalence, and diet

T. S. Edrington, R. L. Farrow, V. Sperandio, D. T. Hughes, T. E. Lawrence, T. R. Callaway, R. C. Anderson, D. J. Nisbet

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Acyl-homoserine-lactone autoinducer (AHL) produced by nonenterohemorrhagic Escherichia coli species in cattle appears to be required for enterohemorrhagic E. coli (EHEC) colonization of the gastrointestinal tract (GIT). The current research aimed to examine the effect of season, diet, EHEC shedding, and location within the GIT on AHL prevalence in the ruminant. Luminal content samples were collected from the rumen and rectum of feedlot cattle at slaughter in the spring, summer, fall, and winter for culture of E. coli O157:H7 and AHL determination. During the spring collection, samples were additionally collected from the cecum and small intestine, but these samples all were AHL negative and therefore not examined again. To assess the influence of diet on AHL prevalence, 14 lambs were fed either 100% forage or 80% concentrate diets and experimentally inoculated with EHEC. At 8 days after infection, all the lambs were killed, and necropsies were taken, with luminal contents collected from the GIT. The collections from the feedlot cattle had AHL in 100% of the rumen content samples from the spring, summer, and fall, but not in any of the winter samples. No other GIT samples from feedlot cattle were AHL positive, and all the samples from the sheep study were AHL negative. The cattle seemed to show a weak correlation between ruminal AHL and EHEC prevalence. This research found AHL only in the rumen and not in the lower GIT of feedlot cattle. However, it is unclear whether this is because the pH of the lower gut destroys the AHL or because a lack of certain bacteria in the lower gut producing AHL.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalCurrent Microbiology
Volume58
Issue number3
DOIs
StatePublished - Mar 2009

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Acyl-Butyrolactones
Escherichia coli O157
Diet
Enterohemorrhagic Escherichia coli
Gastrointestinal Tract
Rumen
Lower Gastrointestinal Tract
Cecum
Ruminants
Research
Rectum
Small Intestine

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Acyl-homoserine-lactone autoinducer in the gastrointesinal tract of feedlot cattle and correlation to season, E. Coli O157 : H7 prevalence, and diet. / Edrington, T. S.; Farrow, R. L.; Sperandio, V.; Hughes, D. T.; Lawrence, T. E.; Callaway, T. R.; Anderson, R. C.; Nisbet, D. J.

In: Current Microbiology, Vol. 58, No. 3, 03.2009, p. 227-232.

Research output: Contribution to journalArticle

Edrington, T. S. ; Farrow, R. L. ; Sperandio, V. ; Hughes, D. T. ; Lawrence, T. E. ; Callaway, T. R. ; Anderson, R. C. ; Nisbet, D. J. / Acyl-homoserine-lactone autoinducer in the gastrointesinal tract of feedlot cattle and correlation to season, E. Coli O157 : H7 prevalence, and diet. In: Current Microbiology. 2009 ; Vol. 58, No. 3. pp. 227-232.
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abstract = "Acyl-homoserine-lactone autoinducer (AHL) produced by nonenterohemorrhagic Escherichia coli species in cattle appears to be required for enterohemorrhagic E. coli (EHEC) colonization of the gastrointestinal tract (GIT). The current research aimed to examine the effect of season, diet, EHEC shedding, and location within the GIT on AHL prevalence in the ruminant. Luminal content samples were collected from the rumen and rectum of feedlot cattle at slaughter in the spring, summer, fall, and winter for culture of E. coli O157:H7 and AHL determination. During the spring collection, samples were additionally collected from the cecum and small intestine, but these samples all were AHL negative and therefore not examined again. To assess the influence of diet on AHL prevalence, 14 lambs were fed either 100{\%} forage or 80{\%} concentrate diets and experimentally inoculated with EHEC. At 8 days after infection, all the lambs were killed, and necropsies were taken, with luminal contents collected from the GIT. The collections from the feedlot cattle had AHL in 100{\%} of the rumen content samples from the spring, summer, and fall, but not in any of the winter samples. No other GIT samples from feedlot cattle were AHL positive, and all the samples from the sheep study were AHL negative. The cattle seemed to show a weak correlation between ruminal AHL and EHEC prevalence. This research found AHL only in the rumen and not in the lower GIT of feedlot cattle. However, it is unclear whether this is because the pH of the lower gut destroys the AHL or because a lack of certain bacteria in the lower gut producing AHL.",
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