Acylation of proteins with myristic acid occurs cotranslationally

Celeste Wilcox, Jing Shan Hu, Eric N. Olson

Research output: Contribution to journalArticle

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Abstract

Several proteins ofviral and cellular origin are acylated with myristic acid early during their biogenesis. To investigate the possibility that myristylation occurred cotranslationally, the BC3Hl muscle cell line, which contains a broad array of myristylated proteins, was pulse-labeled with [3H]myristic acid. Nascent polypeptide chains covalently associated with transfer RNA were isolated subsequently by ion-exchange chromatography. [3H]Myristate was attached to nascent chains through an amide linkage and was identified by thin-layer chromatography after its release from nascent chains by acid methanolysis. Inhibition of cellular protein synthesis with puromycin resulted in cessation of [3H]myristate-labeling of nascent chains, in agreement with the dependence of this modification on protein synthesis in vivo. These data represent a direct demonstration that myristylation of proteins is a cotranslational modification.

Original languageEnglish (US)
Pages (from-to)1275-1278
Number of pages4
JournalScience
Volume238
Issue number4831
StatePublished - 1987

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Acylation
Myristic Acid
Proteins
Puromycin
Protein Array Analysis
Ion Exchange Chromatography
Thin Layer Chromatography
Transfer RNA
Amides
Muscle Cells
Cell Line
Peptides
Acids

ASJC Scopus subject areas

  • General

Cite this

Acylation of proteins with myristic acid occurs cotranslationally. / Wilcox, Celeste; Hu, Jing Shan; Olson, Eric N.

In: Science, Vol. 238, No. 4831, 1987, p. 1275-1278.

Research output: Contribution to journalArticle

Wilcox, C, Hu, JS & Olson, EN 1987, 'Acylation of proteins with myristic acid occurs cotranslationally', Science, vol. 238, no. 4831, pp. 1275-1278.
Wilcox, Celeste ; Hu, Jing Shan ; Olson, Eric N. / Acylation of proteins with myristic acid occurs cotranslationally. In: Science. 1987 ; Vol. 238, No. 4831. pp. 1275-1278.
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