Adaptive effects of 30-night wear of hyper-O2 transmissible contact lenses on bacterial binding and corneal epithelium: A 1-year clinical trial

David H. Ren, Kazuaki Yamamoto, Patrick M. Ladage, Michael Molai, Ling Li, Walter M Petroll, James V. Jester, Harrison D Cavanagh

Research output: Contribution to journalArticle

117 Scopus citations

Abstract

Objective: To determine effects of lens type and oxygen transmissibility on human corneal epithelium during extended wear (EW). Design: Prospective, randomized, double-masked, single-center, parallel treatment groups, 1-year clinical trial. Participants: One hundred seventy-eight patients completed the study: (1) high-O2 Soft lens (6-night [N] EW) (n = 27); (2) hyper-O2 soft lens (6N-EW, n = 33) or (30N-EW, n = 66); and (3) hyper-O2 rigid gas-permeable lens (RGP) (30N-EW, n = 52). Intervention: Irrigation chamber to collect exfoliated corneal surface cells, confocal microscopy, and tear collection at baseline, 1, 3, 6, 9, 12 months of EW. Main Outcome Measures: (1) Pseudomonas aeruginosa (PA) binding to exfoliated corneal surface cells; (2) central epithelial thickness; (3) superficial epithelial cell area; (4) epithelial surface cell exfoliation; and (5) tear lactate dehydrogenase. Results: Quantitative evidence demonstrated increased binding of PA to human exfoliated corneal epithelial cells during the first 3 months of soft lens EW; the control high-O2 test lens showed significantly higher bacterial binding (P < 0.05). Binding activity gradually decreased thereafter and returned to baseline after 9 and 12 months. The corneal epithelium demonstrated enlargement of surface cell size, thinning of central epithelium, and a significant decrease in surface cell shedding (P < 0.05). Remarkably, there was subsequent partial adaptive recovery in cell shedding and epithelial thickness but not surface cell size. There was no significant difference between 6N and 30N continuous wear of the hyper-O2 soft lens for all outcome measures. Importantly, hyper-O2 RGP lens wear did not show significantly increased PA binding during 1 year. Conclusions: This study establishes three important new findings: (1) hyper-O2 soft lens EW produces significantly less PA binding than the lower O2 soft lens with no significant difference in PA binding with 6N versus 30N EW of the hyper-O2 soft lens; (2) there is a remarkable adaptive recovery after 6 months with all soft lens wear with gradual return to prelens PA binding levels and partial recovery of other outcome measures for all test lenses EW except surface cell size; (3) 30N EW of the hyper-O2 RGP lens produced no significant increases in PA binding over 1 year. Taken together, these results suggest that introduction of new hyper-O2 transmissible lens materials into clinical use may offer safer EW, and future epidemiologic studies of ulcerative infectious keratitis should consider both lens type and time in lens EW in any incidence/risk analysis.

Original languageEnglish (US)
Pages (from-to)27-39
Number of pages13
JournalOphthalmology
Volume109
Issue number1
DOIs
StatePublished - Jan 12 2002

ASJC Scopus subject areas

  • Ophthalmology

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