Adaptive responses in hepatic and intestinal cholesterogenesis following ileal resection in the rat

H. J. Weis, J. M. Dietschy

Research output: Contribution to journalArticle

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Abstract

Groups of rats were prepared with a complete resection of either the jejunum or the ileum. Rates of cholesterol synthesis in the liver and small bowel, and fecal excretion of neutral and acidic sterols were then measured approximately 53 days after surgery and compared with similar measurements made in control animals that had had transection and reanastomosis of the intestine. Following resection of the ileum the fecal excretion of neutral sterols (cholesterol + coprostanol) increased from 2.18 to 4.12 mg/day-1 x 100 g-1. In addition, the taurocholic acid pool decreased from 11.3 to 3.9 mg/100 mg-1 and the half life for this bile acid declined from 2.8 to 0.4 days; hence, the metabolic production rate of taurocholic acid increased from 2.71 to 6.70 mg/day-1 x 100 mg-1. Coincidental with this accentuated turnover of both neutral and acidic sterols, the rate of de novo synthesis of cholesterol in the liver and small bowel, as judged by the rate of incorporation of [2 14C]acetate into digitonin precipitable sterols, increased to a nearly identical degree in the animals with ileal resection. This was accomplished in the liver solely by an increase in the rate of synthesis per unit weight of tissue while in the intestine there was both an increase in the weight of the jejunum and an increase in the rate of cholesterol synthesis per unit weight of tissue. In contrast to these striking changes in sterol balance in animals with ileectomy, the experimental group with jejunectomy did not differ significantly from control animals in rates of hepatic and intestinal cholesterol synthesis or in rates of neutral and acidic sterol excretion. Hence, it is apparent from these studies that following resection of the ileum in the rat there is increased endogenous synthesis of cholesterol that fully compensates for the augmented excretion of sterols which follows this operation so that the circulating serum cholesterol pool remains essentially unchanged.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalEuropean Journal of Clinical Investigation
Volume4
Issue number1
StatePublished - 1974

Fingerprint

Sterols
Rats
Cholesterol
Liver
Animals
Ileum
Taurocholic Acid
Jejunum
Weights and Measures
Intestines
Cholestanol
Tissue
Digitonin
Bile Acids and Salts
Ambulatory Surgical Procedures
Surgery
Half-Life
Acetates
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Adaptive responses in hepatic and intestinal cholesterogenesis following ileal resection in the rat. / Weis, H. J.; Dietschy, J. M.

In: European Journal of Clinical Investigation, Vol. 4, No. 1, 1974, p. 33-41.

Research output: Contribution to journalArticle

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