Adding Value to Liver (and Allograft) Biopsy Evaluation Using a Combination of Multiplex Quantum Dot Immunostaining, High-Resolution Whole-Slide Digital Imaging, and Automated Image Analysis

Kumiko Isse, Kedar Grama, Isaac Morse Abbott, Andrew Lesniak, John G. Lunz, William M.F. Lee, Susan Specht, Natasha Corbitt, Yoshiaki Mizuguchi, Badrinath Roysam, A. J. Demetris

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

Various technologies including nucleic acid, protein, and metabolic array analyses of blood, liver tissue, and bile are emerging as powerful tools in the study of hepatic pathophysiology. The entire lexicon of liver disease, however, has been written using classical hematoxylin-eosin staining and light microscopic examination. The authors' goal is to develop new tools to enhance histopathologic examination of liver tissue that would enrich the information gained from liver biopsy analysis, enable quantitative analysis, and bridge the gap between various " -omics" tools and interpretation of routine liver biopsy results. This article describes the progress achieved during the past 2 years in developing multiplex quantum dot (nanoparticle) staining and combining it with high-resolution whole-slide imaging using a slide scanner equipped with filters to capture 9 distinct fluorescent signals for multiple antigens. The authors first focused on precise characterization of leukocyte subsets, but soon realized that the data generated were beyond the practical limits that could be properly evaluated, analyzed, and interpreted visually by a pathologist. Therefore, the authors collaborated with the open source FARSIGHT image analysis project (http://www.farsight-toolkit.org). FARSIGHT's goal is to develop and disseminate the next-generation toolkit of automated image analysis methods to enable quantification of molecular biomarkers on a cell-by-cell basis from multiparameter images. The resulting data can be used for histocytometric studies of the complex and dynamic tissue microenvironments that are of biomedical interest. The authors envisage that these tools will eventually be incorporated into the routine practice of surgical pathology and precipitate a revolution in the specialty.

Original languageEnglish (US)
Pages (from-to)669-685
Number of pages17
JournalClinics in Liver Disease
Volume14
Issue number4
DOIs
StatePublished - Nov 2010

Keywords

  • Automated image analysis
  • Digital pathology
  • Liver allograft pathology
  • Liver biopsy
  • Multiplex immunostaining

ASJC Scopus subject areas

  • Hepatology

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