Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay

Ferid Murad, Alfred G. Gilman

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. 32P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of 3H-labeled guanosine 3′,5′-monophosphate.

Original languageEnglish (US)
Pages (from-to)397-400
Number of pages4
JournalBBA - General Subjects
Volume252
Issue number2
DOIs
StatePublished - Nov 12 1971

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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