Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay

Ferid Murad, Alfred G. Gilman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. 32P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of 3H-labeled guanosine 3′,5′-monophosphate.

Original languageEnglish (US)
Pages (from-to)397-400
Number of pages4
JournalBBA - General Subjects
Volume252
Issue number2
DOIs
StatePublished - Nov 12 1971

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Guanosine Monophosphate
Protein Binding
Adenosine
Assays
Muscle Proteins
Guanosine
Muscle
Skeletal Muscle
Nucleotides
Urine
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate : A simultaneous protein binding assay. / Murad, Ferid; Gilman, Alfred G.

In: BBA - General Subjects, Vol. 252, No. 2, 12.11.1971, p. 397-400.

Research output: Contribution to journalArticle

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