Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay

Ferid Murad; Alfred G. Gilman

doi:10.1016/0304-4165(71)90020-1

Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay

Ferid Murad, Alfred G. Gilman

Pharmacology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. ³²P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of ³H-labeled guanosine 3′,5′-monophosphate.

Original language	English (US)
Pages (from-to)	397-400
Number of pages	4
Journal	BBA - General Subjects
Volume	252
Issue number	2
DOIs	https://doi.org/10.1016/0304-4165(71)90020-1
State	Published - Nov 12 1971

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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title = "Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay",

abstract = "The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. 32P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of 3H-labeled guanosine 3′,5′-monophosphate.",

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T2 - A simultaneous protein binding assay

AU - Murad, Ferid

AU - Gilman, Alfred G.

PY - 1971/11/12

Y1 - 1971/11/12

N2 - The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. 32P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of 3H-labeled guanosine 3′,5′-monophosphate.

AB - The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. 32P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of 3H-labeled guanosine 3′,5′-monophosphate.

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Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay

Abstract

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