Adenosine

A mediator of the sleep-inducing effects of prolonged wakefulness

Tarja Porkka-Heiskanen, Robert E. Strecker, Mahesh Thakkar, Alvhild A. Bjørkum, Robert W. Greene, Robert W. McCarley

Research output: Contribution to journalArticle

779 Citations (Scopus)

Abstract

Both subjective and electroencephalographic arousal diminish as a function of the duration of prior wakefulness. Data reported here suggest that the major criteria for a neural sleep factor mediating the somnogenic effects of prolonged wakefulness are satisfied by adenosine, a neuromodulator whose extracellular concentration increases with brain metabolism and which, in vitro, inhibits basal forebrain cholinergic neurons. In vivo microdialysis measurements in freely behaving cats showed that adenosine extracellular concentrations in the basal forebrain cholinergic region increased during spontaneous wakefulness as contrasted with slow wave sleep; exhibited progressive increases during sustained, prolonged wakefulness; and declined slowly during recovery sleep. Furthermore, the sleep-wakefulness profile occurring after prolonged wakefulness was mimicked by increased extracellular adenosine induced by microdialysis perfusion of an adenosine transport inhibitor in the cholinergic basal forebrain but not by perfusion in a control noncholinergic region.

Original languageEnglish (US)
Pages (from-to)1265-1267
Number of pages3
JournalScience
Volume276
Issue number5316
DOIs
StatePublished - May 23 1997

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Wakefulness
Adenosine
Sleep
Microdialysis
Cholinergic Agents
Perfusion
Cholinergic Neurons
Arousal
Neurotransmitter Agents
Cats
Brain
Basal Forebrain

ASJC Scopus subject areas

  • General

Cite this

Porkka-Heiskanen, T., Strecker, R. E., Thakkar, M., Bjørkum, A. A., Greene, R. W., & McCarley, R. W. (1997). Adenosine: A mediator of the sleep-inducing effects of prolonged wakefulness. Science, 276(5316), 1265-1267. https://doi.org/10.1126/science.276.5316.1265

Adenosine : A mediator of the sleep-inducing effects of prolonged wakefulness. / Porkka-Heiskanen, Tarja; Strecker, Robert E.; Thakkar, Mahesh; Bjørkum, Alvhild A.; Greene, Robert W.; McCarley, Robert W.

In: Science, Vol. 276, No. 5316, 23.05.1997, p. 1265-1267.

Research output: Contribution to journalArticle

Porkka-Heiskanen, T, Strecker, RE, Thakkar, M, Bjørkum, AA, Greene, RW & McCarley, RW 1997, 'Adenosine: A mediator of the sleep-inducing effects of prolonged wakefulness', Science, vol. 276, no. 5316, pp. 1265-1267. https://doi.org/10.1126/science.276.5316.1265
Porkka-Heiskanen T, Strecker RE, Thakkar M, Bjørkum AA, Greene RW, McCarley RW. Adenosine: A mediator of the sleep-inducing effects of prolonged wakefulness. Science. 1997 May 23;276(5316):1265-1267. https://doi.org/10.1126/science.276.5316.1265
Porkka-Heiskanen, Tarja ; Strecker, Robert E. ; Thakkar, Mahesh ; Bjørkum, Alvhild A. ; Greene, Robert W. ; McCarley, Robert W. / Adenosine : A mediator of the sleep-inducing effects of prolonged wakefulness. In: Science. 1997 ; Vol. 276, No. 5316. pp. 1265-1267.
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