Adenosine nucleotides in bile

Ravi S. Chari, S. M. Schutz, J. E. Haebig, Gayle H. Shimokura, Peter B. Cotton, J. Gregory Fitz, William C. Meyers

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Activation of purinergic receptors by ATP stimulates Cl- efflux in biliary epithelial cells. To determine whether purinergic agonists are present under physiological conditions, we have assayed mammalian bile for nucleotides and assessed whether hepatoma and cholangiocarcinoma cell lines are capable of nucleotide release. Bile samples were collected from human, rat, and pig donors and assayed for nucleotide concentrations by luminometry. ATP, ADP, and AMP were present in bile from each species, and the average total nucleotide concentration in human bile was 5.21 ± 0.91 μM (n = 16). In an in vitro model of HTC rat hepatoma cells or Mz-ChA-1 cholangiocarcinoma cells on a superfused column, nucleotides were present in the effluent from each cell type. Addition of α,β-methyleneadenosine 5'-diphosphate (50 μM) to inhibit 5'-nucleotidase activity increased AMP concentrations two- to threefold. Exposure to forskolin (100 μM) or ionomycin (2 μM) stimulated nucleotide release from cholangiocarcinoma but not hepatoma cells. These studies indicate that adenosine nucleotides are present in bile in concentrations sufficient to activate purinergic receptors. Purinergic receptor activation by local nucleotide release might constitute an autocrine and/or paracrine mechanism for modulation of biliary secretion.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume270
Issue number2 33-2
StatePublished - 1996

Fingerprint

Bile
Adenosine
Nucleotides
Purinergic Receptors
Cholangiocarcinoma
Hepatocellular Carcinoma
Adenosine Monophosphate
Purinergic Agonists
Adenosine Triphosphate
5'-Nucleotidase
Ionomycin
Diphosphates
Colforsin
Adenosine Diphosphate
Swine
Epithelial Cells
Cell Line

Keywords

  • adenosine triphosphate
  • bile duct
  • liver
  • purinergic receptors

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Chari, R. S., Schutz, S. M., Haebig, J. E., Shimokura, G. H., Cotton, P. B., Fitz, J. G., & Meyers, W. C. (1996). Adenosine nucleotides in bile. American Journal of Physiology - Gastrointestinal and Liver Physiology, 270(2 33-2).

Adenosine nucleotides in bile. / Chari, Ravi S.; Schutz, S. M.; Haebig, J. E.; Shimokura, Gayle H.; Cotton, Peter B.; Fitz, J. Gregory; Meyers, William C.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 270, No. 2 33-2, 1996.

Research output: Contribution to journalArticle

Chari, RS, Schutz, SM, Haebig, JE, Shimokura, GH, Cotton, PB, Fitz, JG & Meyers, WC 1996, 'Adenosine nucleotides in bile', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 270, no. 2 33-2.
Chari RS, Schutz SM, Haebig JE, Shimokura GH, Cotton PB, Fitz JG et al. Adenosine nucleotides in bile. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1996;270(2 33-2).
Chari, Ravi S. ; Schutz, S. M. ; Haebig, J. E. ; Shimokura, Gayle H. ; Cotton, Peter B. ; Fitz, J. Gregory ; Meyers, William C. / Adenosine nucleotides in bile. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1996 ; Vol. 270, No. 2 33-2.
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