Adenosine reduces cortical neuronal injury induced by oxygen or glucose deprivation in vitro

Mark P. Goldberg; Hannelore Monyer; John H. Weiss; Dennis W. Choi

doi:10.1016/0304-3940(88)90547-2

Adenosine reduces cortical neuronal injury induced by oxygen or glucose deprivation in vitro

Mark P. Goldberg, Hannelore Monyer, John H. Weiss, Dennis W. Choi

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

The endogenous neuromodulatory purine, adenosine, substantially attenuated neuronal degeneration when added to dissociated cortical cell cultures acutely deprived of either oxygen or glucose. The protective effect of adenosine was concentration-dependent between 30 and 1000 μM (EC₅₀ about 100 μM), and could be mimicked by the stable adenosine analogue N⁶-cyclohexyladenosine (10 μM). Unlike postsynaptic glutamate receptor antagonists, which also block these forms of neuronal injury, adenosine did not alter the neurotoxicity of exogenously applied glutamate.

Original language	English (US)
Pages (from-to)	323-327
Number of pages	5
Journal	Neuroscience letters
Volume	89
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(88)90547-2
State	Published - Jul 8 1988

Keywords

Adenosine
Cell culture
Cerebral cortex
Cyclohexyladenosine
Glutamate
Hypoglycemia
Hypoxia

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(88)90547-2

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title = "Adenosine reduces cortical neuronal injury induced by oxygen or glucose deprivation in vitro",

abstract = "The endogenous neuromodulatory purine, adenosine, substantially attenuated neuronal degeneration when added to dissociated cortical cell cultures acutely deprived of either oxygen or glucose. The protective effect of adenosine was concentration-dependent between 30 and 1000 μM (EC50 about 100 μM), and could be mimicked by the stable adenosine analogue N6-cyclohexyladenosine (10 μM). Unlike postsynaptic glutamate receptor antagonists, which also block these forms of neuronal injury, adenosine did not alter the neurotoxicity of exogenously applied glutamate.",

keywords = "Adenosine, Cell culture, Cerebral cortex, Cyclohexyladenosine, Glutamate, Hypoglycemia, Hypoxia",

author = "Goldberg, {Mark P.} and Hannelore Monyer and Weiss, {John H.} and Choi, {Dennis W.}",

note = "Funding Information: We thank V. Viseskul for expert assistance with cell cultures. This research was supported by NIH Grant NS12151, and by a grant from the Hereditary Disease Foundation. Postdoctoral fellowship support was provided by NIH Epilepsy Training Grant NS07820 (M.P.G.), the Deutsche Forschungsgemeinschaft (H.M.), and the American Academy of Neurology (J.H.W.).",

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T1 - Adenosine reduces cortical neuronal injury induced by oxygen or glucose deprivation in vitro

AU - Goldberg, Mark P.

AU - Monyer, Hannelore

AU - Weiss, John H.

AU - Choi, Dennis W.

N1 - Funding Information: We thank V. Viseskul for expert assistance with cell cultures. This research was supported by NIH Grant NS12151, and by a grant from the Hereditary Disease Foundation. Postdoctoral fellowship support was provided by NIH Epilepsy Training Grant NS07820 (M.P.G.), the Deutsche Forschungsgemeinschaft (H.M.), and the American Academy of Neurology (J.H.W.).

PY - 1988/7/8

Y1 - 1988/7/8

N2 - The endogenous neuromodulatory purine, adenosine, substantially attenuated neuronal degeneration when added to dissociated cortical cell cultures acutely deprived of either oxygen or glucose. The protective effect of adenosine was concentration-dependent between 30 and 1000 μM (EC50 about 100 μM), and could be mimicked by the stable adenosine analogue N6-cyclohexyladenosine (10 μM). Unlike postsynaptic glutamate receptor antagonists, which also block these forms of neuronal injury, adenosine did not alter the neurotoxicity of exogenously applied glutamate.

AB - The endogenous neuromodulatory purine, adenosine, substantially attenuated neuronal degeneration when added to dissociated cortical cell cultures acutely deprived of either oxygen or glucose. The protective effect of adenosine was concentration-dependent between 30 and 1000 μM (EC50 about 100 μM), and could be mimicked by the stable adenosine analogue N6-cyclohexyladenosine (10 μM). Unlike postsynaptic glutamate receptor antagonists, which also block these forms of neuronal injury, adenosine did not alter the neurotoxicity of exogenously applied glutamate.

KW - Adenosine

KW - Cell culture

KW - Cerebral cortex

KW - Cyclohexyladenosine

KW - Glutamate

KW - Hypoglycemia

KW - Hypoxia

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ER -

Adenosine reduces cortical neuronal injury induced by oxygen or glucose deprivation in vitro

Abstract

Keywords

ASJC Scopus subject areas

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