Adherence of Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses

Hongwei Ren, Walter M Petroll, James V. Jester, Harrison D Cavanagh, William D. Mathers, Joseph A. Bonanno, Robert H. Kennedy

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Purpose: Previous studies have shown that contact lens oxygen transmissibility correlates with binding of Pseudomonas aeruginosa to the rabbit cornea after overnight lens wear. Studies of human lens wear stratified by oxygen transmissibility will be required to validate these animal results. In humans, bacterial binding to shed cells obtained through corneal irrigation cytology may provide an indirect measure of in vitro binding. The purpose of this study was to establish the relationship between binding to shed cells and to the residual corneal surface in an animal model of lens wear prior to initiation of human studies. Methods: The test contact lenses used were: rigid lens A (Dk/L=10x10-9 [cm/sec][mL O2/mL mmHg]); rigid lens B (Dk/L=97); soft lens A (Dk/L=9); soft lens B (Dk/L=20); and, soft lens C (Dk/L=39). There were six rabbits in each group, except for the soft lens C group, which had seven rabbits. After overnight lens wear, the corneal surface was irrigated with a corneal irrigation chamber to collect surface cells before exposure to a bacterial suspension (1 x 107 CFU/mL) for 30 minutes. The number of bacteria adherent to the residual corneal surface was then assessed by CFU determination. Cells collected from the corneal surface (9 mL) were incubated with 1 mL bacterial suspension containing 108 (CFU/mL) for 30 minutes. The number of bacteria adherent to shed cells was assessed by staining with acridine orange and direct counting by epifluorescence microscopy. Results: The differences in the number of bacteria adhering to shed epithelial cells between the treated and the control eyes were 2.90± 1.20 and 0.23±0.41 for rigid lenses A and B, respectively, and 6.97± 1.54, 3.67±2.32, and 0.90±1.45 (bacteria/cell) for soft lenses A, B, and C, respectively. Overnight contact lens wear induced a significant increase in bacterial binding to shed corneal epithelial cells for rigid lens A end for soft lenses A and B. There were significant differences among lens groups (P=0.00017, ANOVA), with significant differences between rigid lenses A and B, soft lenses A and C, and soft lenses B and C. The binding of bacteria to shed cells was significantly correlated with the binding of bacteria to the residual corneal surface, both confirming and extending previous results (R=0.78, P<0.001). Conclusion: These results demonstrate a positive correlation between P. aeruginosa adherence to shed corneal cells and to the residual corneal surface in the rabbit eye following contact lens wear. In light of the results from prior animal studies, examination of the behavior of P. aeruginosa binding to exfoliated cells appears to be a promising and valid method for future assessment of similar lens-induced increases in bacterial binding in prospective human clinical studies.

Original languageEnglish (US)
Pages (from-to)63-68
Number of pages6
JournalCLAO Journal
Volume23
Issue number1
StatePublished - Jan 1997

Fingerprint

Contact Lenses
Pseudomonas aeruginosa
Lenses
Epithelial Cells
Rabbits
Bacteria
Suspensions
Oxygen
Acridine Orange

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Adherence of Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses. / Ren, Hongwei; Petroll, Walter M; Jester, James V.; Cavanagh, Harrison D; Mathers, William D.; Bonanno, Joseph A.; Kennedy, Robert H.

In: CLAO Journal, Vol. 23, No. 1, 01.1997, p. 63-68.

Research output: Contribution to journalArticle

Ren, Hongwei ; Petroll, Walter M ; Jester, James V. ; Cavanagh, Harrison D ; Mathers, William D. ; Bonanno, Joseph A. ; Kennedy, Robert H. / Adherence of Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses. In: CLAO Journal. 1997 ; Vol. 23, No. 1. pp. 63-68.
@article{d3dac22c5a964528a0af9be365b1ec1a,
title = "Adherence of Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses",
abstract = "Purpose: Previous studies have shown that contact lens oxygen transmissibility correlates with binding of Pseudomonas aeruginosa to the rabbit cornea after overnight lens wear. Studies of human lens wear stratified by oxygen transmissibility will be required to validate these animal results. In humans, bacterial binding to shed cells obtained through corneal irrigation cytology may provide an indirect measure of in vitro binding. The purpose of this study was to establish the relationship between binding to shed cells and to the residual corneal surface in an animal model of lens wear prior to initiation of human studies. Methods: The test contact lenses used were: rigid lens A (Dk/L=10x10-9 [cm/sec][mL O2/mL mmHg]); rigid lens B (Dk/L=97); soft lens A (Dk/L=9); soft lens B (Dk/L=20); and, soft lens C (Dk/L=39). There were six rabbits in each group, except for the soft lens C group, which had seven rabbits. After overnight lens wear, the corneal surface was irrigated with a corneal irrigation chamber to collect surface cells before exposure to a bacterial suspension (1 x 107 CFU/mL) for 30 minutes. The number of bacteria adherent to the residual corneal surface was then assessed by CFU determination. Cells collected from the corneal surface (9 mL) were incubated with 1 mL bacterial suspension containing 108 (CFU/mL) for 30 minutes. The number of bacteria adherent to shed cells was assessed by staining with acridine orange and direct counting by epifluorescence microscopy. Results: The differences in the number of bacteria adhering to shed epithelial cells between the treated and the control eyes were 2.90± 1.20 and 0.23±0.41 for rigid lenses A and B, respectively, and 6.97± 1.54, 3.67±2.32, and 0.90±1.45 (bacteria/cell) for soft lenses A, B, and C, respectively. Overnight contact lens wear induced a significant increase in bacterial binding to shed corneal epithelial cells for rigid lens A end for soft lenses A and B. There were significant differences among lens groups (P=0.00017, ANOVA), with significant differences between rigid lenses A and B, soft lenses A and C, and soft lenses B and C. The binding of bacteria to shed cells was significantly correlated with the binding of bacteria to the residual corneal surface, both confirming and extending previous results (R=0.78, P<0.001). Conclusion: These results demonstrate a positive correlation between P. aeruginosa adherence to shed corneal cells and to the residual corneal surface in the rabbit eye following contact lens wear. In light of the results from prior animal studies, examination of the behavior of P. aeruginosa binding to exfoliated cells appears to be a promising and valid method for future assessment of similar lens-induced increases in bacterial binding in prospective human clinical studies.",
author = "Hongwei Ren and Petroll, {Walter M} and Jester, {James V.} and Cavanagh, {Harrison D} and Mathers, {William D.} and Bonanno, {Joseph A.} and Kennedy, {Robert H.}",
year = "1997",
month = "1",
language = "English (US)",
volume = "23",
pages = "63--68",
journal = "Eye and Contact Lense",
issn = "1542-2321",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Adherence of Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses

AU - Ren, Hongwei

AU - Petroll, Walter M

AU - Jester, James V.

AU - Cavanagh, Harrison D

AU - Mathers, William D.

AU - Bonanno, Joseph A.

AU - Kennedy, Robert H.

PY - 1997/1

Y1 - 1997/1

N2 - Purpose: Previous studies have shown that contact lens oxygen transmissibility correlates with binding of Pseudomonas aeruginosa to the rabbit cornea after overnight lens wear. Studies of human lens wear stratified by oxygen transmissibility will be required to validate these animal results. In humans, bacterial binding to shed cells obtained through corneal irrigation cytology may provide an indirect measure of in vitro binding. The purpose of this study was to establish the relationship between binding to shed cells and to the residual corneal surface in an animal model of lens wear prior to initiation of human studies. Methods: The test contact lenses used were: rigid lens A (Dk/L=10x10-9 [cm/sec][mL O2/mL mmHg]); rigid lens B (Dk/L=97); soft lens A (Dk/L=9); soft lens B (Dk/L=20); and, soft lens C (Dk/L=39). There were six rabbits in each group, except for the soft lens C group, which had seven rabbits. After overnight lens wear, the corneal surface was irrigated with a corneal irrigation chamber to collect surface cells before exposure to a bacterial suspension (1 x 107 CFU/mL) for 30 minutes. The number of bacteria adherent to the residual corneal surface was then assessed by CFU determination. Cells collected from the corneal surface (9 mL) were incubated with 1 mL bacterial suspension containing 108 (CFU/mL) for 30 minutes. The number of bacteria adherent to shed cells was assessed by staining with acridine orange and direct counting by epifluorescence microscopy. Results: The differences in the number of bacteria adhering to shed epithelial cells between the treated and the control eyes were 2.90± 1.20 and 0.23±0.41 for rigid lenses A and B, respectively, and 6.97± 1.54, 3.67±2.32, and 0.90±1.45 (bacteria/cell) for soft lenses A, B, and C, respectively. Overnight contact lens wear induced a significant increase in bacterial binding to shed corneal epithelial cells for rigid lens A end for soft lenses A and B. There were significant differences among lens groups (P=0.00017, ANOVA), with significant differences between rigid lenses A and B, soft lenses A and C, and soft lenses B and C. The binding of bacteria to shed cells was significantly correlated with the binding of bacteria to the residual corneal surface, both confirming and extending previous results (R=0.78, P<0.001). Conclusion: These results demonstrate a positive correlation between P. aeruginosa adherence to shed corneal cells and to the residual corneal surface in the rabbit eye following contact lens wear. In light of the results from prior animal studies, examination of the behavior of P. aeruginosa binding to exfoliated cells appears to be a promising and valid method for future assessment of similar lens-induced increases in bacterial binding in prospective human clinical studies.

AB - Purpose: Previous studies have shown that contact lens oxygen transmissibility correlates with binding of Pseudomonas aeruginosa to the rabbit cornea after overnight lens wear. Studies of human lens wear stratified by oxygen transmissibility will be required to validate these animal results. In humans, bacterial binding to shed cells obtained through corneal irrigation cytology may provide an indirect measure of in vitro binding. The purpose of this study was to establish the relationship between binding to shed cells and to the residual corneal surface in an animal model of lens wear prior to initiation of human studies. Methods: The test contact lenses used were: rigid lens A (Dk/L=10x10-9 [cm/sec][mL O2/mL mmHg]); rigid lens B (Dk/L=97); soft lens A (Dk/L=9); soft lens B (Dk/L=20); and, soft lens C (Dk/L=39). There were six rabbits in each group, except for the soft lens C group, which had seven rabbits. After overnight lens wear, the corneal surface was irrigated with a corneal irrigation chamber to collect surface cells before exposure to a bacterial suspension (1 x 107 CFU/mL) for 30 minutes. The number of bacteria adherent to the residual corneal surface was then assessed by CFU determination. Cells collected from the corneal surface (9 mL) were incubated with 1 mL bacterial suspension containing 108 (CFU/mL) for 30 minutes. The number of bacteria adherent to shed cells was assessed by staining with acridine orange and direct counting by epifluorescence microscopy. Results: The differences in the number of bacteria adhering to shed epithelial cells between the treated and the control eyes were 2.90± 1.20 and 0.23±0.41 for rigid lenses A and B, respectively, and 6.97± 1.54, 3.67±2.32, and 0.90±1.45 (bacteria/cell) for soft lenses A, B, and C, respectively. Overnight contact lens wear induced a significant increase in bacterial binding to shed corneal epithelial cells for rigid lens A end for soft lenses A and B. There were significant differences among lens groups (P=0.00017, ANOVA), with significant differences between rigid lenses A and B, soft lenses A and C, and soft lenses B and C. The binding of bacteria to shed cells was significantly correlated with the binding of bacteria to the residual corneal surface, both confirming and extending previous results (R=0.78, P<0.001). Conclusion: These results demonstrate a positive correlation between P. aeruginosa adherence to shed corneal cells and to the residual corneal surface in the rabbit eye following contact lens wear. In light of the results from prior animal studies, examination of the behavior of P. aeruginosa binding to exfoliated cells appears to be a promising and valid method for future assessment of similar lens-induced increases in bacterial binding in prospective human clinical studies.

UR - http://www.scopus.com/inward/record.url?scp=0031021156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031021156&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 63

EP - 68

JO - Eye and Contact Lense

JF - Eye and Contact Lense

SN - 1542-2321

IS - 1

ER -