Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats

F. Hoentjen, S. L. Tonkonogy, B. Liu, R. B. Sartor, J. D. Taurog, L. A. Dieleman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

HLA-B27 transgenic (TG) rats develop spontaneous colitis when colonized with intestinal bacteria, whereas athymic nude (rnu/rnu) HLA-B27 TG rats remain disease free. The present study was designed to determine whether or not HLA-B27 expression on T cells is required for development of colitis after transfer of mesenteric lymph node (MLN) cells into rnu/rnu HLA-B27 recipients. Athymic nontransgenic (non-TG) and HLA-B27 TG recipients received MLN cells from either TG or non-TG rnu/+ heterozygous donor rats that contain T cells. HLA-B27 TG rnu/rnu recipients receiving either non-TG or TG MLN cells developed severe colitis and had higher caecal MPO and IL-1β levels, and their MLN cells produced more IFN-γ and less IL-10 after in vitro stimulation with caecal bacterial lysate compared to rnu/rnu non-TG recipients that remained disease free after receiving either TG or non-TG cells. Interestingly, proliferating donor TG T cells were detectable one week after adoptive transfer into rnu/rnu TG recipients but not after transfer into non-TG recipients. T cells from either non-TG or TG donors induce colitis in rnu/rnu TG but not in non-TG rats, suggesting that activation of effector T cells by other cell types that express HLA-B27 is pivotal for the pathogenesis of colitis in this model.

Original languageEnglish (US)
Pages (from-to)474-483
Number of pages10
JournalClinical and Experimental Immunology
Volume143
Issue number3
DOIs
StatePublished - Mar 2006

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Nude Rats
Transgenic Rats
HLA-B27 Antigen
Adoptive Transfer
Colitis
Lymph Nodes
T-Lymphocytes
Interleukin-1
Interleukin-10
Bacteria

Keywords

  • Commensal bacteria
  • HLA-B27
  • Nude
  • T cells
  • Transgenic rats

ASJC Scopus subject areas

  • Immunology

Cite this

Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats. / Hoentjen, F.; Tonkonogy, S. L.; Liu, B.; Sartor, R. B.; Taurog, J. D.; Dieleman, L. A.

In: Clinical and Experimental Immunology, Vol. 143, No. 3, 03.2006, p. 474-483.

Research output: Contribution to journalArticle

Hoentjen, F. ; Tonkonogy, S. L. ; Liu, B. ; Sartor, R. B. ; Taurog, J. D. ; Dieleman, L. A. / Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats. In: Clinical and Experimental Immunology. 2006 ; Vol. 143, No. 3. pp. 474-483.
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