Advances in fluorescence labeling strategies for dynamic cellular imaging

Kevin M. Dean, Amy E. Palmer

Research output: Contribution to journalReview articlepeer-review

252 Scopus citations

Abstract

Synergistic advances in optical physics, probe design, molecular biology, labeling techniques and computational analysis have propelled fluorescence imaging into new realms of spatiotemporal resolution and sensitivity. This review aims to discuss advances in fluorescent probes and live-cell labeling strategies, two areas that remain pivotal for future advances in imaging technology. Fluorescent protein- and bio-orthogonal-based methods for protein and RNA imaging are discussed as well as emerging bioengineering techniques that enable their expression at specific genomic loci (for example, CRISPR and TALENs). Important attributes that contribute to the success of each technique are emphasized, providing a guideline for future advances in dynamic live-cell imaging.

Original languageEnglish (US)
Pages (from-to)512-523
Number of pages12
JournalNature chemical biology
Volume10
Issue number7
DOIs
StatePublished - Jul 2014
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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