TY - JOUR
T1 - Advances in fluorescence labeling strategies for dynamic cellular imaging
AU - Dean, Kevin M.
AU - Palmer, Amy E.
PY - 2014/7
Y1 - 2014/7
N2 - Synergistic advances in optical physics, probe design, molecular biology, labeling techniques and computational analysis have propelled fluorescence imaging into new realms of spatiotemporal resolution and sensitivity. This review aims to discuss advances in fluorescent probes and live-cell labeling strategies, two areas that remain pivotal for future advances in imaging technology. Fluorescent protein- and bio-orthogonal-based methods for protein and RNA imaging are discussed as well as emerging bioengineering techniques that enable their expression at specific genomic loci (for example, CRISPR and TALENs). Important attributes that contribute to the success of each technique are emphasized, providing a guideline for future advances in dynamic live-cell imaging.
AB - Synergistic advances in optical physics, probe design, molecular biology, labeling techniques and computational analysis have propelled fluorescence imaging into new realms of spatiotemporal resolution and sensitivity. This review aims to discuss advances in fluorescent probes and live-cell labeling strategies, two areas that remain pivotal for future advances in imaging technology. Fluorescent protein- and bio-orthogonal-based methods for protein and RNA imaging are discussed as well as emerging bioengineering techniques that enable their expression at specific genomic loci (for example, CRISPR and TALENs). Important attributes that contribute to the success of each technique are emphasized, providing a guideline for future advances in dynamic live-cell imaging.
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U2 - 10.1038/nchembio.1556
DO - 10.1038/nchembio.1556
M3 - Review article
C2 - 24937069
AN - SCOPUS:84902773575
SN - 1552-4450
VL - 10
SP - 512
EP - 523
JO - Nature chemical biology
JF - Nature chemical biology
IS - 7
ER -