Age-related analysis of EcoRI generated satellite DNA-containing chromatin of rat liver

Pratima Chaurasia, Shibani Mukherjee, M. K. Thakur

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

EcoRI digestion of nuclei and their subsequent lysis with EDTA solubilizes 45% and 36% of chromatin DNA from the liver of young (18 ± 2 weeks) and old (100 ± 5 weeks) rats, respectively. After hybridization with 185 bp rat satellite I DNA, these soluble fractions are found to be enriched in specific DNA sequences such as satellite DNA. Besides regular repeat pattern, a major portion of the satellite chromatin forms higher order organization. Digestion kinetics confirms condensation of satellite DNA-containing chromatin similar to that of bulk chromatin in old age. Furthermore, densitometric scanning of the slot-blot of soluble chromatin fractions reveals loss of satellite DNA in the old. However, an increase in the linker histone H1 and its subfraction H10 in the satellite DNA-enriched fraction of chromatin from old rats suggests greater compaction. These results provide the first evidence that the satellite DNA-containing chromatin differs in the liver of young and old rats.

Original languageEnglish (US)
Pages (from-to)1261-1270
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume40
Issue number6
StatePublished - Dec 1 1996

Keywords

  • Aging
  • Histone
  • Rat liver
  • Satellite DNA
  • Soluble chromatin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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