Abstract
EcoRI digestion of nuclei and their subsequent lysis with EDTA solubilizes 45% and 36% of chromatin DNA from the liver of young (18 ± 2 weeks) and old (100 ± 5 weeks) rats, respectively. After hybridization with 185 bp rat satellite I DNA, these soluble fractions are found to be enriched in specific DNA sequences such as satellite DNA. Besides regular repeat pattern, a major portion of the satellite chromatin forms higher order organization. Digestion kinetics confirms condensation of satellite DNA-containing chromatin similar to that of bulk chromatin in old age. Furthermore, densitometric scanning of the slot-blot of soluble chromatin fractions reveals loss of satellite DNA in the old. However, an increase in the linker histone H1 and its subfraction H10 in the satellite DNA-enriched fraction of chromatin from old rats suggests greater compaction. These results provide the first evidence that the satellite DNA-containing chromatin differs in the liver of young and old rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1261-1270 |
| Number of pages | 10 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 40 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1996 |
Keywords
- Aging
- Histone
- Rat liver
- Satellite DNA
- Soluble chromatin
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics