Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies

Ze Xu Jiao, Min Xu, Teresa K. Woodruff

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Purpose: To confirm that aneuploidy candidate genes are detectable in the first polar body (PB1) of MII oocytes and to investigate the age-dependent molecular changes in PB1. Methods: Aged (12-to 15-mo-old) and young (2-mo-old) mice were administered pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG). MII oocytes were obtained and the first PB was removed. mRNA from each PB and its sibling oocyte was reverse transcribed. Real-time PCR was performed to quantify the expression of six genes (BUB1, CDC20, Filia, MCAK, SGOL1, SMC1A) in single PB. Results: We first demonstrated that detection and quantification of transcripts associated with aneuploidy in single mouse oocyte and sibling PB1 is possible and the relative abundance of mRNA transcripts in a single PB faithfully reflects the relative abundance of that transcript in its sibling oocyte. We further found that transcript levels were significantly lower in aged PBs compared with young PBs (P<0.05). Conclusions: Our results suggest that the detection and analysis of polar body mRNA may provide insight in age-related aneuploidy in oocyte. This analysis is a novel concept to investigate the genesis of chromosome abnormality and could potentially assist in the characterization of mechanisms underlying key molecular origin of female meiotic aneuploidy, which would be of great scientific and clinical value.

Original languageEnglish (US)
Pages (from-to)731-737
Number of pages7
JournalJournal of assisted reproduction and genetics
Volume31
Issue number6
DOIs
StatePublished - Jun 2014
Externally publishedYes

Keywords

  • Age
  • Aneuploidy
  • Genes expression
  • Infertility
  • Meiosis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

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