Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts.

R. C. Eberhart, M. S. Munro, G. B. Williams, P. V. Kulkarni, W. A. Shannon, B. E. Brink, W. J. Fry

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The short-term albumin affinity and thrombo-resistance of a polyether polyurethane vascular graft have been improved. The method is based on the C18 alkylation of the polymer. Thrombus formation by a planimetric technique and albumin retention on wire-reinforced polyurethane tubes, both C18 alkylated and untreated, were measured in short-term (4-h) exposure at femoral arterial sites in the dog. 125I-Albumin was preabsorbed on tubes and then exposed to blood for successive 2-h periods. Albumin uptake on alkylated tubes prior to blood exposure and retention following 2 h of blood exposure were significantly greater than on controls. Following a fast desorption phase in blood, the remaining albumin was more slowly desorbed from alkylated than from control tubes. Reincubation with albumin and blood reexposure produced a similar tendency, suggesting blood conditioning does not reduce the albumin affinity-enhancing property of C18 alkylation in the short term. Blood-preconditioning experiments suggested endogenous albumin has a high affinity for the C18-alkylated surface. Scanning electron microscopic examination showed thrombus and platelet densities were higher on control than on alkylated surfaces. These results suggest in vivo albumin affinity is increased for C18-alkylated polyurethane, which may be linked to decreased thrombus formation on these surfaces.

Original languageEnglish (US)
Pages (from-to)375-382
Number of pages8
JournalArtificial Organs
Volume11
Issue number5
StatePublished - Oct 1987

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Polyurethanes
Grafts
Adsorption
Blood Vessels
Albumins
Blood
Transplants
Alkylation
Thrombosis
Polyethers
Platelets
Desorption
Microscopic examination
Thigh
Wire
Scanning
Polymers
Blood Platelets
Electrons
Dogs

ASJC Scopus subject areas

  • Biophysics

Cite this

Eberhart, R. C., Munro, M. S., Williams, G. B., Kulkarni, P. V., Shannon, W. A., Brink, B. E., & Fry, W. J. (1987). Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts. Artificial Organs, 11(5), 375-382.

Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts. / Eberhart, R. C.; Munro, M. S.; Williams, G. B.; Kulkarni, P. V.; Shannon, W. A.; Brink, B. E.; Fry, W. J.

In: Artificial Organs, Vol. 11, No. 5, 10.1987, p. 375-382.

Research output: Contribution to journalArticle

Eberhart, RC, Munro, MS, Williams, GB, Kulkarni, PV, Shannon, WA, Brink, BE & Fry, WJ 1987, 'Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts.', Artificial Organs, vol. 11, no. 5, pp. 375-382.
Eberhart RC, Munro MS, Williams GB, Kulkarni PV, Shannon WA, Brink BE et al. Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts. Artificial Organs. 1987 Oct;11(5):375-382.
Eberhart, R. C. ; Munro, M. S. ; Williams, G. B. ; Kulkarni, P. V. ; Shannon, W. A. ; Brink, B. E. ; Fry, W. J. / Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts. In: Artificial Organs. 1987 ; Vol. 11, No. 5. pp. 375-382.
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